Experiment: P. simiae Grant Run MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11095 and PS417_11100 are separated by 93 nucleotides PS417_11100 and PS417_11105 are separated by 2 nucleotides PS417_11105 and PS417_11110 are separated by 1 nucleotides PS417_11110 and PS417_11115 are separated by 50 nucleotides
PS417_11095: PS417_11095 - fatty acid desaturase, at 2,378,965 to 2,380,059
_11095
PS417_11100: PS417_11100 - MocE, at 2,380,153 to 2,380,473
_11100
PS417_11105: PS417_11105 - pyridine nucleotide-disulfide oxidoreductase, at 2,380,476 to 2,381,675
_11105
PS417_11110: PS417_11110 - xylose isomerase, at 2,381,677 to 2,382,495
_11110
PS417_11115: PS417_11115 - peroxidase, at 2,382,546 to 2,383,502
_11115
Position (kb)
2380
2381
2382 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2379.606 kb on + strand, within PS417_11095 at 2379.637 kb on + strand, within PS417_11095 at 2379.698 kb on + strand, within PS417_11095 at 2379.778 kb on + strand at 2379.779 kb on - strand, within PS417_11095 at 2379.779 kb on - strand, within PS417_11095 at 2379.802 kb on - strand, within PS417_11095 at 2379.805 kb on + strand, within PS417_11095 at 2379.821 kb on - strand at 2379.821 kb on - strand, within PS417_11095 at 2379.842 kb on - strand, within PS417_11095 at 2379.842 kb on - strand, within PS417_11095 at 2379.911 kb on - strand, within PS417_11095 at 2379.931 kb on + strand, within PS417_11095 at 2379.931 kb on + strand, within PS417_11095 at 2379.932 kb on - strand, within PS417_11095 at 2380.137 kb on - strand at 2380.237 kb on + strand, within PS417_11100 at 2380.238 kb on - strand, within PS417_11100 at 2380.238 kb on - strand, within PS417_11100 at 2381.578 kb on + strand at 2381.578 kb on + strand at 2381.578 kb on + strand at 2381.578 kb on + strand at 2381.578 kb on + strand at 2381.791 kb on + strand, within PS417_11110 at 2381.951 kb on + strand at 2381.952 kb on - strand, within PS417_11110 at 2382.133 kb on + strand, within PS417_11110 at 2382.134 kb on - strand, within PS417_11110 at 2382.366 kb on + strand, within PS417_11110 at 2382.367 kb on - strand, within PS417_11110 at 2382.367 kb on - strand, within PS417_11110
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 0.1 remove 2,379,606 + PS417_11095 0.59 +1.3 2,379,637 + PS417_11095 0.61 -0.6 2,379,698 + PS417_11095 0.67 -0.6 2,379,778 + -0.1 2,379,779 - PS417_11095 0.74 +0.3 2,379,779 - PS417_11095 0.74 +0.2 2,379,802 - PS417_11095 0.76 +0.1 2,379,805 + PS417_11095 0.77 +2.5 2,379,821 - +0.5 2,379,821 - PS417_11095 0.78 +0.5 2,379,842 - PS417_11095 0.80 -2.8 2,379,842 - PS417_11095 0.80 -1.1 2,379,911 - PS417_11095 0.86 -0.5 2,379,931 + PS417_11095 0.88 -0.2 2,379,931 + PS417_11095 0.88 +0.1 2,379,932 - PS417_11095 0.88 +0.4 2,380,137 - +1.1 2,380,237 + PS417_11100 0.26 +0.3 2,380,238 - PS417_11100 0.26 +0.3 2,380,238 - PS417_11100 0.26 +0.0 2,381,578 + +0.9 2,381,578 + -0.1 2,381,578 + -0.7 2,381,578 + +0.6 2,381,578 + -1.6 2,381,791 + PS417_11110 0.14 +3.3 2,381,951 + +2.0 2,381,952 - PS417_11110 0.34 -1.1 2,382,133 + PS417_11110 0.56 -1.1 2,382,134 - PS417_11110 0.56 +0.2 2,382,366 + PS417_11110 0.84 -0.6 2,382,367 - PS417_11110 0.84 +1.1 2,382,367 - PS417_11110 0.84 +0.4
Or see this region's nucleotide sequence