Experiment: P. simiae Grant Run MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00535 and PS417_00540 are separated by 80 nucleotides PS417_00540 and PS417_00545 are separated by 136 nucleotides PS417_00545 and PS417_00550 are separated by 263 nucleotides PS417_00550 and PS417_00555 are separated by 317 nucleotides
PS417_00535: PS417_00535 - Holliday junction resolvase, at 108,229 to 108,537
_00535
PS417_00540: PS417_00540 - hypothetical protein, at 108,618 to 108,938
_00540
PS417_00545: PS417_00545 - hypothetical protein, at 109,075 to 109,260
_00545
PS417_00550: PS417_00550 - hypothetical protein, at 109,524 to 109,739
_00550
PS417_00555: PS417_00555 - acetyl-CoA hydrolase, at 110,057 to 111,550
_00555
Position (kb)
109
110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 108.576 kb on - strand at 108.623 kb on + strand at 108.953 kb on - strand at 108.953 kb on - strand at 108.953 kb on - strand at 108.965 kb on - strand at 109.003 kb on + strand at 109.003 kb on + strand at 109.004 kb on - strand at 109.006 kb on + strand at 109.006 kb on + strand at 109.007 kb on - strand at 109.033 kb on + strand at 109.237 kb on - strand, within PS417_00545 at 109.239 kb on - strand, within PS417_00545 at 109.239 kb on - strand, within PS417_00545 at 109.324 kb on + strand at 109.324 kb on + strand at 109.324 kb on + strand at 109.325 kb on - strand at 109.349 kb on + strand at 109.372 kb on + strand at 109.373 kb on - strand at 109.693 kb on - strand, within PS417_00550 at 109.730 kb on + strand at 109.731 kb on - strand at 109.768 kb on + strand at 109.773 kb on - strand at 109.773 kb on - strand at 109.781 kb on - strand at 109.823 kb on + strand at 109.823 kb on + strand at 109.861 kb on + strand at 109.863 kb on + strand at 109.872 kb on + strand at 109.873 kb on - strand at 109.942 kb on + strand at 109.942 kb on + strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 110.051 kb on + strand at 110.052 kb on - strand at 110.052 kb on - strand at 110.163 kb on + strand at 110.203 kb on + strand at 110.204 kb on - strand at 110.204 kb on - strand at 110.322 kb on - strand, within PS417_00555 at 110.463 kb on + strand, within PS417_00555 at 110.463 kb on + strand, within PS417_00555 at 110.464 kb on - strand, within PS417_00555 at 110.464 kb on - strand, within PS417_00555 at 110.500 kb on + strand, within PS417_00555 at 110.501 kb on - strand, within PS417_00555 at 110.501 kb on - strand, within PS417_00555 at 110.504 kb on + strand, within PS417_00555 at 110.505 kb on - strand, within PS417_00555 at 110.568 kb on + strand, within PS417_00555 at 110.568 kb on + strand, within PS417_00555 at 110.568 kb on + strand, within PS417_00555 at 110.569 kb on - strand, within PS417_00555 at 110.569 kb on - strand, within PS417_00555 at 110.694 kb on + strand, within PS417_00555 at 110.695 kb on - strand, within PS417_00555
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 0.1 remove 108,576 - -1.4 108,623 + +1.4 108,953 - -2.2 108,953 - -0.4 108,953 - +0.3 108,965 - -3.0 109,003 + -0.5 109,003 + -0.0 109,004 - -0.9 109,006 + +1.7 109,006 + +1.0 109,007 - -0.4 109,033 + -2.2 109,237 - PS417_00545 0.87 +1.2 109,239 - PS417_00545 0.88 +0.1 109,239 - PS417_00545 0.88 -0.2 109,324 + -3.0 109,324 + -0.6 109,324 + +0.2 109,325 - -1.2 109,349 + -0.1 109,372 + +0.9 109,373 - +1.2 109,693 - PS417_00550 0.78 -3.2 109,730 + -0.1 109,731 - -0.3 109,768 + +0.5 109,773 - +0.3 109,773 - +0.5 109,781 - -0.2 109,823 + -1.0 109,823 + +0.6 109,861 + -1.7 109,863 + -0.1 109,872 + -0.1 109,873 - +1.6 109,942 + -0.1 109,942 + -0.6 109,943 - -1.6 109,943 - -0.3 109,943 - -0.8 109,943 - -2.1 109,943 - +0.9 109,943 - -0.4 109,943 - +0.8 109,943 - -1.2 109,943 - +0.4 109,943 - -1.7 110,051 + +0.7 110,052 - -1.1 110,052 - -0.4 110,163 + -0.0 110,203 + -0.5 110,204 - +0.3 110,204 - -1.0 110,322 - PS417_00555 0.18 -0.2 110,463 + PS417_00555 0.27 +0.2 110,463 + PS417_00555 0.27 +2.1 110,464 - PS417_00555 0.27 +0.5 110,464 - PS417_00555 0.27 -1.4 110,500 + PS417_00555 0.30 -0.4 110,501 - PS417_00555 0.30 +0.6 110,501 - PS417_00555 0.30 -0.3 110,504 + PS417_00555 0.30 +0.6 110,505 - PS417_00555 0.30 +0.7 110,568 + PS417_00555 0.34 +0.8 110,568 + PS417_00555 0.34 +0.6 110,568 + PS417_00555 0.34 -0.2 110,569 - PS417_00555 0.34 +1.3 110,569 - PS417_00555 0.34 +0.6 110,694 + PS417_00555 0.43 -0.8 110,695 - PS417_00555 0.43 +0.4
Or see this region's nucleotide sequence