Experiment: P. simiae Grant Run MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00115 and PS417_00120 are separated by 159 nucleotides PS417_00120 and PS417_00125 overlap by 1 nucleotides PS417_00125 and PS417_00130 are separated by 36 nucleotides
PS417_00115: PS417_00115 - quinone oxidoreductase, at 24,371 to 25,348
_00115
PS417_00120: PS417_00120 - coproporphyrinogen III oxidase, at 25,508 to 26,440
_00120
PS417_00125: PS417_00125 - shikimate dehydrogenase, at 26,440 to 27,258
_00125
PS417_00130: PS417_00130 - SulP family inorganic anion transporter, at 27,295 to 28,887
_00130
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 24.526 kb on + strand, within PS417_00115 at 24.526 kb on + strand, within PS417_00115 at 24.527 kb on - strand, within PS417_00115 at 24.527 kb on - strand, within PS417_00115 at 24.543 kb on + strand, within PS417_00115 at 24.570 kb on + strand, within PS417_00115 at 24.571 kb on - strand, within PS417_00115 at 24.709 kb on - strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.943 kb on - strand, within PS417_00115 at 24.943 kb on - strand, within PS417_00115 at 25.065 kb on + strand, within PS417_00115 at 25.212 kb on + strand, within PS417_00115 at 25.213 kb on - strand, within PS417_00115 at 25.247 kb on - strand, within PS417_00115 at 25.287 kb on + strand at 25.287 kb on + strand at 25.287 kb on + strand at 25.288 kb on - strand at 25.288 kb on - strand at 25.296 kb on + strand at 25.296 kb on + strand at 25.296 kb on + strand at 25.297 kb on - strand at 25.395 kb on + strand at 25.395 kb on + strand at 25.396 kb on - strand at 25.396 kb on - strand at 25.438 kb on + strand at 26.536 kb on + strand, within PS417_00125 at 27.190 kb on + strand at 27.263 kb on - strand at 27.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 0.1 remove 24,526 + PS417_00115 0.16 +0.0 24,526 + PS417_00115 0.16 -1.1 24,527 - PS417_00115 0.16 -2.0 24,527 - PS417_00115 0.16 +2.2 24,543 + PS417_00115 0.18 +0.3 24,570 + PS417_00115 0.20 -0.7 24,571 - PS417_00115 0.20 +0.1 24,709 - PS417_00115 0.35 -0.2 24,942 + PS417_00115 0.58 -0.7 24,942 + PS417_00115 0.58 +0.3 24,942 + PS417_00115 0.58 -1.7 24,943 - PS417_00115 0.58 +2.3 24,943 - PS417_00115 0.58 -0.2 25,065 + PS417_00115 0.71 +0.0 25,212 + PS417_00115 0.86 +0.2 25,213 - PS417_00115 0.86 -1.0 25,247 - PS417_00115 0.90 -0.9 25,287 + +0.1 25,287 + +0.3 25,287 + -0.5 25,288 - +1.0 25,288 - -0.5 25,296 + +0.5 25,296 + +0.7 25,296 + -1.7 25,297 - -0.0 25,395 + +0.2 25,395 + -0.0 25,396 - -1.4 25,396 - +0.7 25,438 + -1.7 26,536 + PS417_00125 0.12 +1.7 27,190 + +0.7 27,263 - -2.9 27,263 - -0.1
Or see this region's nucleotide sequence