Experiment: P. simiae Grant Run MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02440 and PS417_02445 are separated by 51 nucleotides PS417_02445 and PS417_02450 are separated by 108 nucleotides PS417_02450 and PS417_02455 are separated by 194 nucleotides
PS417_02440: PS417_02440 - flagellar motor protein MotB, at 559,057 to 560,076
_02440
PS417_02445: PS417_02445 - GTPase RsgA, at 560,128 to 561,159
_02445
PS417_02450: PS417_02450 - oligoribonuclease, at 561,268 to 561,810
_02450
PS417_02455: PS417_02455 - putative transporter, required for glycine utilization <small>(from data)</small>, at 562,005 to 562,619
_02455
Position (kb) 560
561
562 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 559.231 kb on + strand, within PS417_02440 at 559.232 kb on - strand, within PS417_02440 at 559.676 kb on - strand, within PS417_02440 at 559.968 kb on + strand, within PS417_02440 at 559.969 kb on - strand, within PS417_02440 at 560.085 kb on + strand at 560.132 kb on + strand at 560.132 kb on + strand at 561.192 kb on + strand at 561.209 kb on + strand at 561.240 kb on + strand at 561.241 kb on - strand at 561.241 kb on - strand at 561.241 kb on - strand at 562.020 kb on + strand at 562.020 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 10 remove 559,231 + PS417_02440 0.17 +2.1 559,232 - PS417_02440 0.17 +2.0 559,676 - PS417_02440 0.61 +1.3 559,968 + PS417_02440 0.89 +4.0 559,969 - PS417_02440 0.89 -0.3 560,085 + -3.1 560,132 + -1.7 560,132 + +0.2 561,192 + -1.2 561,209 + +0.1 561,240 + -1.0 561,241 - +0.2 561,241 - +0.3 561,241 - -0.6 562,020 + +1.1 562,020 + -1.3
Or see this region's nucleotide sequence