Experiment: P. simiae Grant Run MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21855 and PS417_21860 overlap by 4 nucleotides PS417_21860 and PS417_21865 overlap by 4 nucleotides PS417_21865 and PS417_21870 are separated by 232 nucleotides PS417_21870 and PS417_21875 are separated by 0 nucleotides PS417_21875 and PS417_21880 overlap by 9 nucleotides
PS417_21855: PS417_21855 - hypothetical protein, at 4,745,409 to 4,746,110
_21855
PS417_21860: PS417_21860 - hypothetical protein, at 4,746,107 to 4,746,430
_21860
PS417_21865: PS417_21865 - hypothetical protein, at 4,746,427 to 4,746,750
_21865
PS417_21870: PS417_21870 - glycerophosphodiester phosphodiesterase, at 4,746,983 to 4,748,110
_21870
PS417_21875: PS417_21875 - AraC family transcriptional regulator, at 4,748,111 to 4,748,764
_21875
PS417_21880: PS417_21880 - fatty acid hydroxylase, at 4,748,756 to 4,749,619
_21880
Position (kb)
4746
4747
4748
4749 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4746.134 kb on + strand at 4746.134 kb on + strand at 4746.135 kb on - strand at 4746.135 kb on - strand at 4746.138 kb on - strand at 4746.138 kb on - strand at 4746.148 kb on - strand, within PS417_21860 at 4746.480 kb on + strand at 4746.480 kb on + strand, within PS417_21865 at 4746.481 kb on - strand, within PS417_21865 at 4746.481 kb on - strand, within PS417_21865 at 4746.482 kb on + strand, within PS417_21865 at 4746.482 kb on + strand, within PS417_21865 at 4746.668 kb on + strand, within PS417_21865 at 4746.669 kb on - strand, within PS417_21865 at 4746.753 kb on + strand at 4746.959 kb on + strand at 4746.960 kb on - strand at 4746.982 kb on + strand at 4746.983 kb on - strand at 4747.194 kb on - strand, within PS417_21870 at 4747.231 kb on - strand, within PS417_21870 at 4747.231 kb on - strand, within PS417_21870 at 4747.962 kb on - strand, within PS417_21870 at 4748.100 kb on - strand at 4748.101 kb on + strand at 4748.101 kb on + strand at 4748.101 kb on + strand at 4748.101 kb on + strand at 4748.102 kb on - strand at 4748.102 kb on - strand at 4748.102 kb on - strand at 4748.105 kb on + strand at 4748.105 kb on + strand at 4748.106 kb on - strand at 4748.285 kb on - strand, within PS417_21875 at 4748.342 kb on + strand, within PS417_21875 at 4748.366 kb on - strand, within PS417_21875 at 4748.763 kb on - strand at 4748.763 kb on - strand at 4748.763 kb on - strand at 4748.901 kb on + strand, within PS417_21880 at 4748.973 kb on + strand, within PS417_21880
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 10 remove 4,746,134 + -0.9 4,746,134 + -0.2 4,746,135 - -0.0 4,746,135 - +0.3 4,746,138 - -0.5 4,746,138 - +2.0 4,746,148 - PS417_21860 0.13 -0.3 4,746,480 + -0.8 4,746,480 + PS417_21865 0.16 +0.7 4,746,481 - PS417_21865 0.17 -0.5 4,746,481 - PS417_21865 0.17 -0.4 4,746,482 + PS417_21865 0.17 +0.5 4,746,482 + PS417_21865 0.17 -1.5 4,746,668 + PS417_21865 0.74 -0.5 4,746,669 - PS417_21865 0.75 +0.9 4,746,753 + +1.0 4,746,959 + +0.7 4,746,960 - -0.0 4,746,982 + -2.0 4,746,983 - +0.7 4,747,194 - PS417_21870 0.19 +1.9 4,747,231 - PS417_21870 0.22 -0.5 4,747,231 - PS417_21870 0.22 -1.9 4,747,962 - PS417_21870 0.87 -0.9 4,748,100 - -0.4 4,748,101 + +0.0 4,748,101 + -0.5 4,748,101 + -1.2 4,748,101 + +0.6 4,748,102 - -2.1 4,748,102 - -0.5 4,748,102 - +1.3 4,748,105 + -0.5 4,748,105 + +0.1 4,748,106 - -0.2 4,748,285 - PS417_21875 0.27 -1.1 4,748,342 + PS417_21875 0.35 +0.2 4,748,366 - PS417_21875 0.39 -0.6 4,748,763 - -1.9 4,748,763 - -0.1 4,748,763 - +0.6 4,748,901 + PS417_21880 0.17 -0.6 4,748,973 + PS417_21880 0.25 -0.3
Or see this region's nucleotide sequence