Strain Fitness in Pseudomonas simiae WCS417 around PS417_10580

Experiment: P. simiae Grant Run MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_10575 and PS417_10580 are separated by 18 nucleotidesPS417_10580 and PS417_10585 are separated by 11 nucleotidesPS417_10585 and PS417_10590 are separated by 153 nucleotides PS417_10575: PS417_10575 - DNA alkylation repair protein, at 2,258,388 to 2,259,488 _10575 PS417_10580: PS417_10580 - glutamine synthetase, at 2,259,507 to 2,260,853 _10580 PS417_10585: PS417_10585 - Cro/Cl family transcriptional regulator, at 2,260,865 to 2,261,422 _10585 PS417_10590: PS417_10590 - hypothetical protein, at 2,261,576 to 2,261,884 _10590 Position (kb) 2259 2260 2261Strain fitness (log2 ratio) -3 -2 -1 0 1at 2258.593 kb on + strand, within PS417_10575at 2258.915 kb on - strand, within PS417_10575at 2259.260 kb on - strand, within PS417_10575at 2259.509 kb on - strandat 2260.039 kb on + strand, within PS417_10580at 2260.039 kb on + strand, within PS417_10580at 2260.040 kb on - strand, within PS417_10580at 2260.084 kb on + strandat 2260.085 kb on - strand, within PS417_10580at 2260.244 kb on + strand, within PS417_10580at 2260.245 kb on - strand, within PS417_10580at 2260.319 kb on + strand, within PS417_10580at 2260.408 kb on + strand, within PS417_10580at 2260.627 kb on + strandat 2260.783 kb on + strandat 2260.866 kb on + strandat 2260.866 kb on + strandat 2260.867 kb on - strandat 2260.873 kb on - strandat 2261.154 kb on + strand, within PS417_10585at 2261.155 kb on - strand, within PS417_10585at 2261.218 kb on - strand, within PS417_10585at 2261.223 kb on + strand, within PS417_10585at 2261.224 kb on - strand, within PS417_10585

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Per-strain Table

Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 10
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2,258,593 + PS417_10575 0.19 -0.1
2,258,915 - PS417_10575 0.48 +0.6
2,259,260 - PS417_10575 0.79 -3.4
2,259,509 - -1.3
2,260,039 + PS417_10580 0.39 -1.5
2,260,039 + PS417_10580 0.39 -1.3
2,260,040 - PS417_10580 0.40 -0.9
2,260,084 + -0.5
2,260,085 - PS417_10580 0.43 -1.5
2,260,244 + PS417_10580 0.55 +0.1
2,260,245 - PS417_10580 0.55 -0.4
2,260,319 + PS417_10580 0.60 -0.4
2,260,408 + PS417_10580 0.67 -1.2
2,260,627 + +0.5
2,260,783 + +0.4
2,260,866 + +0.9
2,260,866 + -2.3
2,260,867 - -1.5
2,260,873 - +0.1
2,261,154 + PS417_10585 0.52 +0.1
2,261,155 - PS417_10585 0.52 -0.2
2,261,218 - PS417_10585 0.63 +0.4
2,261,223 + PS417_10585 0.64 -0.3
2,261,224 - PS417_10585 0.64 +0.2

Or see this region's nucleotide sequence