Experiment: P. simiae Grant Run MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01015 and PS417_01020 overlap by 20 nucleotides PS417_01020 and PS417_01025 overlap by 4 nucleotides PS417_01025 and PS417_01030 overlap by 4 nucleotides
PS417_01015: PS417_01015 - polar amino acid ABC transporter permease, at 221,869 to 222,534
_01015
PS417_01020: PS417_01020 - glutamine ABC transporter permease, at 222,515 to 223,174
_01020
PS417_01025: PS417_01025 - glutamine ABC transporter ATP-binding protein, at 223,171 to 223,947
_01025
PS417_01030: PS417_01030 - glutamine ABC transporter substrate-binding protein, at 223,944 to 224,798
_01030
Position (kb) 222
223
224 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 221.588 kb on - strand at 221.694 kb on + strand at 221.694 kb on + strand at 221.694 kb on + strand at 221.694 kb on + strand at 221.695 kb on - strand at 221.695 kb on - strand at 221.695 kb on - strand at 221.695 kb on - strand at 221.844 kb on + strand at 221.889 kb on + strand at 221.889 kb on + strand at 222.040 kb on + strand, within PS417_01015 at 222.091 kb on + strand, within PS417_01015 at 222.385 kb on + strand, within PS417_01015 at 222.400 kb on + strand, within PS417_01015 at 222.400 kb on + strand, within PS417_01015 at 222.401 kb on - strand, within PS417_01015 at 222.412 kb on + strand, within PS417_01015 at 222.415 kb on + strand, within PS417_01015 at 222.415 kb on + strand, within PS417_01015 at 222.415 kb on + strand, within PS417_01015 at 222.416 kb on - strand, within PS417_01015 at 222.416 kb on - strand, within PS417_01015 at 222.416 kb on - strand, within PS417_01015 at 222.424 kb on + strand, within PS417_01015 at 222.424 kb on + strand, within PS417_01015 at 222.589 kb on + strand, within PS417_01020 at 222.636 kb on - strand, within PS417_01020 at 222.689 kb on + strand, within PS417_01020 at 222.731 kb on + strand, within PS417_01020 at 222.731 kb on + strand, within PS417_01020 at 222.731 kb on + strand, within PS417_01020 at 222.732 kb on - strand, within PS417_01020 at 222.917 kb on + strand, within PS417_01020 at 222.923 kb on + strand, within PS417_01020 at 222.924 kb on - strand, within PS417_01020 at 223.067 kb on + strand, within PS417_01020 at 223.067 kb on + strand, within PS417_01020 at 223.068 kb on - strand, within PS417_01020 at 223.068 kb on - strand, within PS417_01020 at 223.086 kb on - strand, within PS417_01020 at 223.114 kb on + strand at 223.115 kb on - strand at 223.435 kb on + strand, within PS417_01025 at 223.944 kb on - strand at 224.134 kb on - strand, within PS417_01030
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 10 remove 221,588 - -0.0 221,694 + -0.6 221,694 + -0.6 221,694 + -0.1 221,694 + -0.5 221,695 - -1.1 221,695 - -4.2 221,695 - -3.0 221,695 - -4.6 221,844 + -2.7 221,889 + +1.7 221,889 + -0.5 222,040 + PS417_01015 0.26 -0.7 222,091 + PS417_01015 0.33 -0.1 222,385 + PS417_01015 0.77 -0.3 222,400 + PS417_01015 0.80 +0.3 222,400 + PS417_01015 0.80 -1.8 222,401 - PS417_01015 0.80 -3.3 222,412 + PS417_01015 0.82 -0.3 222,415 + PS417_01015 0.82 -1.8 222,415 + PS417_01015 0.82 -0.6 222,415 + PS417_01015 0.82 -2.0 222,416 - PS417_01015 0.82 -1.5 222,416 - PS417_01015 0.82 -1.1 222,416 - PS417_01015 0.82 +0.2 222,424 + PS417_01015 0.83 +1.0 222,424 + PS417_01015 0.83 +1.1 222,589 + PS417_01020 0.11 -0.7 222,636 - PS417_01020 0.18 -1.5 222,689 + PS417_01020 0.26 -0.1 222,731 + PS417_01020 0.33 -0.2 222,731 + PS417_01020 0.33 +0.5 222,731 + PS417_01020 0.33 -1.1 222,732 - PS417_01020 0.33 -2.1 222,917 + PS417_01020 0.61 -0.7 222,923 + PS417_01020 0.62 +0.1 222,924 - PS417_01020 0.62 -4.2 223,067 + PS417_01020 0.84 -0.1 223,067 + PS417_01020 0.84 -0.1 223,068 - PS417_01020 0.84 -2.5 223,068 - PS417_01020 0.84 -0.3 223,086 - PS417_01020 0.87 -2.9 223,114 + -0.0 223,115 - -1.6 223,435 + PS417_01025 0.34 -0.9 223,944 - -2.7 224,134 - PS417_01030 0.22 +0.7
Or see this region's nucleotide sequence