Experiment: P. simiae Grant Run MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26055 and PS417_26060 overlap by 4 nucleotides PS417_26060 and PS417_26065 are separated by 100 nucleotides PS417_26065 and PS417_26070 overlap by 4 nucleotides
PS417_26055: PS417_26055 - cyclic nucleotide-binding protein, at 5,629,909 to 5,631,831
_26055
PS417_26060: PS417_26060 - DNA polymerase III subunit epsilon, at 5,631,828 to 5,632,535
_26060
PS417_26065: PS417_26065 - RNA polymerase sigma factor, at 5,632,636 to 5,633,154
_26065
PS417_26070: PS417_26070 - amino acid ABC transporter substrate-binding protein, at 5,633,151 to 5,634,146
_26070
Position (kb) 5632
5633
5634 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5631.641 kb on - strand at 5631.660 kb on + strand at 5631.852 kb on - strand at 5632.000 kb on + strand, within PS417_26060 at 5632.198 kb on + strand, within PS417_26060 at 5632.480 kb on + strand at 5632.480 kb on + strand at 5632.480 kb on + strand at 5632.480 kb on + strand at 5632.481 kb on - strand at 5632.533 kb on + strand at 5632.533 kb on + strand at 5632.534 kb on - strand at 5632.681 kb on + strand at 5632.682 kb on - strand at 5632.858 kb on + strand, within PS417_26065 at 5632.859 kb on - strand, within PS417_26065 at 5632.859 kb on - strand, within PS417_26065 at 5632.888 kb on + strand, within PS417_26065 at 5632.888 kb on + strand, within PS417_26065 at 5633.023 kb on + strand, within PS417_26065 at 5633.024 kb on - strand, within PS417_26065 at 5633.025 kb on + strand, within PS417_26065 at 5633.026 kb on - strand, within PS417_26065 at 5633.126 kb on - strand at 5633.469 kb on + strand, within PS417_26070 at 5633.469 kb on + strand, within PS417_26070 at 5633.469 kb on + strand, within PS417_26070 at 5633.670 kb on + strand, within PS417_26070 at 5633.670 kb on + strand, within PS417_26070 at 5633.671 kb on - strand, within PS417_26070 at 5633.674 kb on + strand, within PS417_26070 at 5633.675 kb on - strand, within PS417_26070 at 5633.675 kb on - strand, within PS417_26070 at 5634.135 kb on - strand at 5634.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 10 remove 5,631,641 - +0.7 5,631,660 + -0.2 5,631,852 - +0.0 5,632,000 + PS417_26060 0.24 -2.9 5,632,198 + PS417_26060 0.52 -0.5 5,632,480 + +2.9 5,632,480 + +0.1 5,632,480 + -1.0 5,632,480 + -0.4 5,632,481 - +0.7 5,632,533 + -1.8 5,632,533 + -0.5 5,632,534 - -0.5 5,632,681 + -0.4 5,632,682 - +1.8 5,632,858 + PS417_26065 0.43 +2.0 5,632,859 - PS417_26065 0.43 -0.6 5,632,859 - PS417_26065 0.43 -1.9 5,632,888 + PS417_26065 0.49 -0.8 5,632,888 + PS417_26065 0.49 -0.3 5,633,023 + PS417_26065 0.75 -0.6 5,633,024 - PS417_26065 0.75 -0.9 5,633,025 + PS417_26065 0.75 +0.3 5,633,026 - PS417_26065 0.75 -2.6 5,633,126 - +0.8 5,633,469 + PS417_26070 0.32 +0.6 5,633,469 + PS417_26070 0.32 -2.0 5,633,469 + PS417_26070 0.32 +0.2 5,633,670 + PS417_26070 0.52 -0.7 5,633,670 + PS417_26070 0.52 +0.1 5,633,671 - PS417_26070 0.52 -1.3 5,633,674 + PS417_26070 0.53 -1.0 5,633,675 - PS417_26070 0.53 -2.3 5,633,675 - PS417_26070 0.53 +1.0 5,634,135 - -2.4 5,634,135 - +1.6
Or see this region's nucleotide sequence