Experiment: P. simiae Grant Run MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16790 and PS417_16795 overlap by 4 nucleotides PS417_16795 and PS417_16800 overlap by 4 nucleotides PS417_16800 and PS417_16805 are separated by 123 nucleotides
PS417_16790: PS417_16790 - ABC transporter permease, at 3,663,076 to 3,663,957
_16790
PS417_16795: PS417_16795 - ABC transporter, at 3,663,954 to 3,664,727
_16795
PS417_16800: PS417_16800 - acyl-CoA synthetase, at 3,664,724 to 3,665,626
_16800
PS417_16805: PS417_16805 - 5,10-methylene tetrahydromethanopterin reductase, at 3,665,750 to 3,667,129
_16805
Position (kb)
3664
3665
3666 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3663.906 kb on - strand at 3663.906 kb on - strand at 3663.977 kb on - strand at 3664.132 kb on - strand, within PS417_16795 at 3664.794 kb on + strand at 3664.794 kb on + strand at 3664.795 kb on - strand at 3664.795 kb on - strand at 3664.795 kb on - strand at 3665.241 kb on + strand, within PS417_16800 at 3665.241 kb on + strand, within PS417_16800 at 3665.241 kb on + strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.242 kb on - strand, within PS417_16800 at 3665.692 kb on + strand at 3665.692 kb on + strand at 3665.692 kb on + strand at 3665.693 kb on - strand at 3665.693 kb on - strand at 3665.778 kb on + strand at 3665.854 kb on - strand at 3665.943 kb on - strand, within PS417_16805 at 3665.943 kb on - strand, within PS417_16805 at 3666.225 kb on + strand, within PS417_16805 at 3666.398 kb on + strand, within PS417_16805 at 3666.624 kb on + strand, within PS417_16805 at 3666.625 kb on - strand, within PS417_16805
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae Grant Run MOI 10 remove 3,663,906 - -1.3 3,663,906 - +0.2 3,663,977 - +0.2 3,664,132 - PS417_16795 0.23 +3.1 3,664,794 + -0.4 3,664,794 + +1.0 3,664,795 - -1.4 3,664,795 - -0.4 3,664,795 - +0.4 3,665,241 + PS417_16800 0.57 -0.7 3,665,241 + PS417_16800 0.57 -2.2 3,665,241 + PS417_16800 0.57 -1.3 3,665,242 - PS417_16800 0.57 +0.3 3,665,242 - PS417_16800 0.57 -1.2 3,665,242 - PS417_16800 0.57 -0.0 3,665,242 - PS417_16800 0.57 +0.2 3,665,242 - PS417_16800 0.57 -0.9 3,665,242 - PS417_16800 0.57 +0.2 3,665,692 + -0.6 3,665,692 + +0.5 3,665,692 + -0.2 3,665,693 - -0.3 3,665,693 - -0.3 3,665,778 + +2.1 3,665,854 - -0.8 3,665,943 - PS417_16805 0.14 +0.0 3,665,943 - PS417_16805 0.14 +0.1 3,666,225 + PS417_16805 0.34 -0.4 3,666,398 + PS417_16805 0.47 -0.3 3,666,624 + PS417_16805 0.63 +0.1 3,666,625 - PS417_16805 0.63 +0.5
Or see this region's nucleotide sequence