Strain Fitness in Pseudomonas simiae WCS417 around PS417_06260

Experiment: P. simiae ORA MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_06250 and PS417_06255 are separated by 45 nucleotidesPS417_06255 and PS417_06260 are separated by 3 nucleotidesPS417_06260 and PS417_06265 are separated by 111 nucleotidesPS417_06265 and PS417_06270 overlap by 4 nucleotides PS417_06250: PS417_06250 - membrane protein, at 1,386,328 to 1,388,715 _06250 PS417_06255: PS417_06255 - Skp, at 1,388,761 to 1,389,264 _06255 PS417_06260: PS417_06260 - UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase, at 1,389,268 to 1,390,323 _06260 PS417_06265: PS417_06265 - 3-hydroxyacyl-ACP dehydratase, at 1,390,435 to 1,390,875 _06265 PS417_06270: PS417_06270 - UDP-N-acetylglucosamine acyltransferase, at 1,390,872 to 1,391,648 _06270 Position (kb) 1389 1390 1391Strain fitness (log2 ratio) -2 -1 0 1at 1388.755 kb on + strandat 1388.766 kb on + strandat 1390.392 kb on + strandat 1390.392 kb on + strandat 1390.392 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. simiae ORA MOI 0.1
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1,388,755 + -1.8
1,388,766 + +0.2
1,390,392 + -1.2
1,390,392 + +0.2
1,390,392 + +0.4

Or see this region's nucleotide sequence