Experiment: P. simiae ORA MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10895 and PS417_10900 are separated by 262 nucleotides PS417_10900 and PS417_10905 are separated by 47 nucleotides PS417_10905 and PS417_10910 overlap by 1 nucleotides
PS417_10895: PS417_10895 - AMP-dependent synthetase, at 2,329,399 to 2,331,099
_10895
PS417_10900: PS417_10900 - ABC transporter substrate-binding protein, at 2,331,362 to 2,332,138
_10900
PS417_10905: PS417_10905 - hypothetical protein, at 2,332,186 to 2,332,812
_10905
PS417_10910: PS417_10910 - FAD-dependent oxidoreductase, at 2,332,812 to 2,333,945
_10910
Position (kb)
2331
2332
2333 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2330.388 kb on + strand, within PS417_10895 at 2330.389 kb on - strand, within PS417_10895 at 2330.426 kb on + strand, within PS417_10895 at 2330.426 kb on + strand, within PS417_10895 at 2330.426 kb on + strand, within PS417_10895 at 2330.427 kb on - strand, within PS417_10895 at 2330.510 kb on + strand, within PS417_10895 at 2330.622 kb on + strand, within PS417_10895 at 2330.623 kb on - strand, within PS417_10895 at 2330.623 kb on - strand, within PS417_10895 at 2330.842 kb on + strand, within PS417_10895 at 2330.858 kb on - strand, within PS417_10895 at 2331.112 kb on + strand at 2331.117 kb on - strand at 2331.117 kb on - strand at 2331.165 kb on + strand at 2331.166 kb on - strand at 2331.166 kb on - strand at 2331.516 kb on - strand, within PS417_10900 at 2331.516 kb on - strand, within PS417_10900 at 2331.568 kb on - strand, within PS417_10900 at 2331.683 kb on + strand, within PS417_10900 at 2331.684 kb on - strand, within PS417_10900 at 2331.687 kb on - strand, within PS417_10900 at 2331.740 kb on + strand, within PS417_10900 at 2331.741 kb on - strand, within PS417_10900 at 2331.863 kb on + strand, within PS417_10900 at 2331.863 kb on + strand at 2331.864 kb on - strand, within PS417_10900 at 2332.127 kb on + strand at 2332.149 kb on - strand at 2332.149 kb on - strand at 2332.179 kb on - strand at 2332.179 kb on - strand at 2332.253 kb on + strand, within PS417_10905 at 2332.253 kb on + strand, within PS417_10905 at 2332.254 kb on - strand, within PS417_10905 at 2332.492 kb on - strand, within PS417_10905 at 2332.548 kb on + strand, within PS417_10905 at 2332.606 kb on + strand, within PS417_10905 at 2332.606 kb on + strand, within PS417_10905 at 2332.607 kb on - strand at 2332.657 kb on + strand, within PS417_10905 at 2332.657 kb on + strand, within PS417_10905 at 2332.658 kb on - strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.689 kb on - strand, within PS417_10905 at 2332.845 kb on - strand at 2333.092 kb on + strand, within PS417_10910 at 2333.093 kb on - strand, within PS417_10910
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 0.1 remove 2,330,388 + PS417_10895 0.58 +0.7 2,330,389 - PS417_10895 0.58 -0.9 2,330,426 + PS417_10895 0.60 -0.7 2,330,426 + PS417_10895 0.60 -1.3 2,330,426 + PS417_10895 0.60 -0.1 2,330,427 - PS417_10895 0.60 -0.1 2,330,510 + PS417_10895 0.65 +0.3 2,330,622 + PS417_10895 0.72 -0.4 2,330,623 - PS417_10895 0.72 -0.4 2,330,623 - PS417_10895 0.72 +0.2 2,330,842 + PS417_10895 0.85 +3.1 2,330,858 - PS417_10895 0.86 -0.4 2,331,112 + -0.5 2,331,117 - +0.2 2,331,117 - +0.1 2,331,165 + +0.3 2,331,166 - -0.5 2,331,166 - +0.2 2,331,516 - PS417_10900 0.20 +1.2 2,331,516 - PS417_10900 0.20 +1.6 2,331,568 - PS417_10900 0.27 +0.7 2,331,683 + PS417_10900 0.41 +0.9 2,331,684 - PS417_10900 0.41 +0.6 2,331,687 - PS417_10900 0.42 +1.2 2,331,740 + PS417_10900 0.49 -1.0 2,331,741 - PS417_10900 0.49 -0.4 2,331,863 + PS417_10900 0.64 +0.5 2,331,863 + +2.7 2,331,864 - PS417_10900 0.65 -0.4 2,332,127 + -0.1 2,332,149 - -1.1 2,332,149 - -1.3 2,332,179 - -0.2 2,332,179 - -1.4 2,332,253 + PS417_10905 0.11 +0.4 2,332,253 + PS417_10905 0.11 +0.4 2,332,254 - PS417_10905 0.11 -0.2 2,332,492 - PS417_10905 0.49 -0.8 2,332,548 + PS417_10905 0.58 +0.7 2,332,606 + PS417_10905 0.67 -0.2 2,332,606 + PS417_10905 0.67 -1.8 2,332,607 - +0.2 2,332,657 + PS417_10905 0.75 -0.4 2,332,657 + PS417_10905 0.75 -0.2 2,332,658 - PS417_10905 0.75 -2.4 2,332,688 + PS417_10905 0.80 +1.3 2,332,688 + PS417_10905 0.80 +0.5 2,332,688 + PS417_10905 0.80 +0.9 2,332,689 - PS417_10905 0.80 +0.2 2,332,845 - -0.8 2,333,092 + PS417_10910 0.25 -0.1 2,333,093 - PS417_10910 0.25 +0.7
Or see this region's nucleotide sequence