Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03925 and PS417_03930 are separated by 191 nucleotides PS417_03930 and PS417_03935 are separated by 61 nucleotides PS417_03935 and PS417_03940 overlap by 4 nucleotides
PS417_03925: PS417_03925 - nicotinate-nucleotide pyrophosphorylase, at 876,190 to 877,038
_03925
PS417_03930: PS417_03930 - thymidine phosphorylase, at 877,230 to 879,353
_03930
PS417_03935: PS417_03935 - N-acetyl-anhydromuranmyl-L-alanine amidase, at 879,415 to 879,978
_03935
PS417_03940: PS417_03940 - membrane protein, at 879,975 to 880,811
_03940
Position (kb) 877
878
879
880 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 876.257 kb on + strand at 876.257 kb on + strand at 876.257 kb on + strand at 876.258 kb on - strand at 876.258 kb on - strand at 876.258 kb on - strand at 876.258 kb on - strand at 876.360 kb on + strand, within PS417_03925 at 876.361 kb on - strand, within PS417_03925 at 876.548 kb on + strand, within PS417_03925 at 876.548 kb on + strand, within PS417_03925 at 876.549 kb on - strand, within PS417_03925 at 876.594 kb on - strand, within PS417_03925 at 877.071 kb on - strand at 877.110 kb on + strand at 877.110 kb on + strand at 877.160 kb on - strand at 877.304 kb on - strand at 877.430 kb on + strand at 877.430 kb on + strand at 877.430 kb on + strand at 877.430 kb on + strand at 877.617 kb on - strand, within PS417_03930 at 877.830 kb on + strand, within PS417_03930 at 878.130 kb on + strand, within PS417_03930 at 878.130 kb on + strand, within PS417_03930 at 878.130 kb on + strand, within PS417_03930 at 878.130 kb on + strand, within PS417_03930 at 878.130 kb on + strand, within PS417_03930 at 878.130 kb on + strand, within PS417_03930 at 878.130 kb on + strand, within PS417_03930 at 878.131 kb on - strand, within PS417_03930 at 878.131 kb on - strand, within PS417_03930 at 878.134 kb on + strand, within PS417_03930 at 878.135 kb on - strand, within PS417_03930 at 878.313 kb on + strand, within PS417_03930 at 878.313 kb on + strand, within PS417_03930 at 878.314 kb on - strand, within PS417_03930 at 878.314 kb on - strand, within PS417_03930 at 878.463 kb on + strand, within PS417_03930 at 878.463 kb on + strand, within PS417_03930 at 878.463 kb on + strand, within PS417_03930 at 878.464 kb on - strand, within PS417_03930 at 879.022 kb on + strand, within PS417_03930 at 879.023 kb on - strand, within PS417_03930 at 879.186 kb on + strand at 879.187 kb on - strand at 879.187 kb on - strand at 879.187 kb on - strand at 879.187 kb on - strand at 879.187 kb on - strand at 879.187 kb on - strand at 879.187 kb on - strand at 879.187 kb on - strand at 879.414 kb on + strand at 879.520 kb on - strand, within PS417_03935 at 879.604 kb on + strand, within PS417_03935 at 879.604 kb on + strand, within PS417_03935 at 879.626 kb on + strand, within PS417_03935 at 879.626 kb on + strand, within PS417_03935 at 879.626 kb on + strand, within PS417_03935 at 879.627 kb on - strand, within PS417_03935 at 880.003 kb on + strand at 880.136 kb on - strand, within PS417_03940 at 880.136 kb on - strand, within PS417_03940 at 880.139 kb on - strand, within PS417_03940 at 880.231 kb on - strand, within PS417_03940 at 880.231 kb on - strand, within PS417_03940 at 880.231 kb on - strand, within PS417_03940 at 880.231 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 876,257 + +1.3 876,257 + +0.8 876,257 + -0.8 876,258 - -0.5 876,258 - -1.2 876,258 - +0.2 876,258 - +2.0 876,360 + PS417_03925 0.20 +1.0 876,361 - PS417_03925 0.20 -0.1 876,548 + PS417_03925 0.42 +1.4 876,548 + PS417_03925 0.42 +1.7 876,549 - PS417_03925 0.42 -1.4 876,594 - PS417_03925 0.48 -1.0 877,071 - -1.0 877,110 + +1.5 877,110 + +0.9 877,160 - -1.6 877,304 - -4.1 877,430 + -1.0 877,430 + -1.5 877,430 + -2.6 877,430 + -1.2 877,617 - PS417_03930 0.18 -1.0 877,830 + PS417_03930 0.28 -3.7 878,130 + PS417_03930 0.42 +0.0 878,130 + PS417_03930 0.42 -3.1 878,130 + PS417_03930 0.42 -3.2 878,130 + PS417_03930 0.42 -4.2 878,130 + PS417_03930 0.42 -1.9 878,130 + PS417_03930 0.42 -2.7 878,130 + PS417_03930 0.42 -3.3 878,131 - PS417_03930 0.42 -2.3 878,131 - PS417_03930 0.42 -3.1 878,134 + PS417_03930 0.43 -1.4 878,135 - PS417_03930 0.43 -4.1 878,313 + PS417_03930 0.51 -3.4 878,313 + PS417_03930 0.51 -0.4 878,314 - PS417_03930 0.51 +0.3 878,314 - PS417_03930 0.51 +0.0 878,463 + PS417_03930 0.58 -3.9 878,463 + PS417_03930 0.58 -3.4 878,463 + PS417_03930 0.58 +0.6 878,464 - PS417_03930 0.58 -4.6 879,022 + PS417_03930 0.84 -0.6 879,023 - PS417_03930 0.84 -4.1 879,186 + -1.6 879,187 - -3.2 879,187 - -2.5 879,187 - -2.1 879,187 - +0.0 879,187 - -1.6 879,187 - -2.0 879,187 - -1.9 879,187 - -4.2 879,414 + -0.7 879,520 - PS417_03935 0.19 -0.2 879,604 + PS417_03935 0.34 +2.6 879,604 + PS417_03935 0.34 +0.8 879,626 + PS417_03935 0.37 +0.2 879,626 + PS417_03935 0.37 +0.3 879,626 + PS417_03935 0.37 -1.7 879,627 - PS417_03935 0.38 +0.7 880,003 + +1.1 880,136 - PS417_03940 0.19 -0.7 880,136 - PS417_03940 0.19 -0.2 880,139 - PS417_03940 0.20 -1.7 880,231 - PS417_03940 0.31 +0.8 880,231 - PS417_03940 0.31 -0.1 880,231 - PS417_03940 0.31 +0.0 880,231 - +1.0
Or see this region's nucleotide sequence