Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00370 and PS417_00375 are separated by 37 nucleotides PS417_00375 and PS417_00380 overlap by 8 nucleotides PS417_00380 and PS417_00385 overlap by 1 nucleotides PS417_00385 and PS417_00390 are separated by 67 nucleotides
PS417_00370: PS417_00370 - hypothetical protein, at 72,707 to 73,426
_00370
PS417_00375: PS417_00375 - membrane protein, at 73,464 to 74,252
_00375
PS417_00380: PS417_00380 - zinc ABC transporter ATP-binding protein, at 74,245 to 75,030
_00380
PS417_00385: PS417_00385 - Fur family transcriptional regulator, at 75,030 to 75,512
_00385
PS417_00390: PS417_00390 - ABC transporter substrate-binding protein, at 75,580 to 76,500
_00390
Position (kb) 74
75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 73.440 kb on + strand at 73.465 kb on + strand at 73.887 kb on - strand, within PS417_00375 at 73.913 kb on - strand, within PS417_00375 at 74.155 kb on + strand, within PS417_00375 at 74.262 kb on + strand at 74.348 kb on + strand, within PS417_00380 at 74.550 kb on - strand at 74.550 kb on - strand, within PS417_00380 at 75.347 kb on - strand, within PS417_00385 at 75.543 kb on - strand at 75.545 kb on + strand at 75.546 kb on - strand at 75.660 kb on - strand at 75.756 kb on + strand, within PS417_00390 at 75.756 kb on + strand, within PS417_00390 at 75.757 kb on - strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.761 kb on - strand, within PS417_00390
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 73,440 + +0.6 73,465 + +0.8 73,887 - PS417_00375 0.54 +0.3 73,913 - PS417_00375 0.57 -1.3 74,155 + PS417_00375 0.88 -0.3 74,262 + -0.8 74,348 + PS417_00380 0.13 -0.6 74,550 - +0.1 74,550 - PS417_00380 0.39 -0.8 75,347 - PS417_00385 0.66 +0.0 75,543 - -2.3 75,545 + -0.0 75,546 - -0.2 75,660 - -0.2 75,756 + PS417_00390 0.19 -0.3 75,756 + PS417_00390 0.19 -0.5 75,757 - PS417_00390 0.19 -1.5 75,760 + PS417_00390 0.20 +0.1 75,760 + PS417_00390 0.20 -1.9 75,761 - PS417_00390 0.20 -2.8
Or see this region's nucleotide sequence