Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03455 and PS417_03460 overlap by 4 nucleotides PS417_03460 and PS417_03465 are separated by 34 nucleotides PS417_03465 and PS417_03470 overlap by 4 nucleotides
PS417_03455: PS417_03455 - type III secretion protein, at 793,973 to 795,037
_03455
PS417_03460: PS417_03460 - type III secretion protein HrpI, at 795,034 to 797,127
_03460
PS417_03465: PS417_03465 - type III secretion protein, at 797,162 to 798,061
_03465
PS417_03470: PS417_03470 - ATP synthase, at 798,058 to 799,416
_03470
Position (kb) 795
796
797
798 Strain fitness (log2 ratio) -2
-1
0
1 at 794.321 kb on + strand, within PS417_03455 at 794.321 kb on + strand, within PS417_03455 at 794.322 kb on - strand, within PS417_03455 at 794.817 kb on + strand at 795.181 kb on - strand at 795.181 kb on - strand at 795.218 kb on - strand at 795.218 kb on - strand at 796.000 kb on + strand, within PS417_03460 at 796.000 kb on + strand, within PS417_03460 at 796.003 kb on + strand, within PS417_03460 at 796.003 kb on + strand, within PS417_03460 at 796.004 kb on - strand, within PS417_03460 at 796.118 kb on - strand, within PS417_03460 at 796.781 kb on - strand, within PS417_03460 at 797.404 kb on - strand, within PS417_03465 at 797.521 kb on + strand, within PS417_03465 at 797.522 kb on - strand, within PS417_03465 at 798.045 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 794,321 + PS417_03455 0.33 -2.1 794,321 + PS417_03455 0.33 +0.6 794,322 - PS417_03455 0.33 -0.6 794,817 + +0.6 795,181 - +1.2 795,181 - -1.8 795,218 - +0.2 795,218 - -0.2 796,000 + PS417_03460 0.46 +0.3 796,000 + PS417_03460 0.46 -0.5 796,003 + PS417_03460 0.46 -1.0 796,003 + PS417_03460 0.46 -2.4 796,004 - PS417_03460 0.46 +0.3 796,118 - PS417_03460 0.52 -1.0 796,781 - PS417_03460 0.83 -1.1 797,404 - PS417_03465 0.27 -0.4 797,521 + PS417_03465 0.40 -2.2 797,522 - PS417_03465 0.40 -0.7 798,045 - -0.8
Or see this region's nucleotide sequence