Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28505 and PS417_28510 are separated by 9 nucleotides PS417_28510 and PS417_28515 are separated by 18 nucleotides PS417_28515 and PS417_28520 overlap by 4 nucleotides
PS417_28505: PS417_28505 - chromosome partitioning protein ParB, at 6,155,933 to 6,156,805
_28505
PS417_28510: PS417_28510 - chromosome partitioning protein, at 6,156,815 to 6,157,612
_28510
PS417_28515: PS417_28515 - 16S rRNA methyltransferase, at 6,157,631 to 6,158,275
_28515
PS417_28520: PS417_28520 - tRNA uridine 5-carboxymethylaminomethyl modification protein, at 6,158,272 to 6,160,164
_28520
Position (kb)
6157
6158
6159 Strain fitness (log2 ratio)
-1
0
1
2 at 6156.817 kb on - strand at 6156.817 kb on - strand at 6156.987 kb on + strand, within PS417_28510 at 6156.987 kb on + strand, within PS417_28510 at 6157.155 kb on + strand, within PS417_28510 at 6157.159 kb on - strand, within PS417_28510 at 6157.159 kb on - strand, within PS417_28510 at 6157.251 kb on + strand, within PS417_28510 at 6157.387 kb on - strand, within PS417_28510 at 6157.387 kb on - strand, within PS417_28510 at 6157.431 kb on + strand, within PS417_28510 at 6157.601 kb on + strand at 6157.650 kb on - strand at 6157.730 kb on + strand, within PS417_28515 at 6158.139 kb on + strand, within PS417_28515 at 6158.139 kb on + strand, within PS417_28515 at 6158.140 kb on - strand, within PS417_28515
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 6,156,817 - -0.3 6,156,817 - -0.6 6,156,987 + PS417_28510 0.22 +0.8 6,156,987 + PS417_28510 0.22 +0.2 6,157,155 + PS417_28510 0.43 -0.1 6,157,159 - PS417_28510 0.43 +2.1 6,157,159 - PS417_28510 0.43 -1.4 6,157,251 + PS417_28510 0.55 -0.1 6,157,387 - PS417_28510 0.72 -1.5 6,157,387 - PS417_28510 0.72 -1.4 6,157,431 + PS417_28510 0.77 -0.3 6,157,601 + -0.7 6,157,650 - -1.1 6,157,730 + PS417_28515 0.15 +0.1 6,158,139 + PS417_28515 0.79 -1.2 6,158,139 + PS417_28515 0.79 +0.1 6,158,140 - PS417_28515 0.79 -1.1
Or see this region's nucleotide sequence