Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28165 and PS417_28170 are separated by 87 nucleotides PS417_28170 and PS417_28175 overlap by 4 nucleotides PS417_28175 and PS417_28180 are separated by 10 nucleotides
PS417_28165: PS417_28165 - GntR family transcriptional regulator, at 6,082,136 to 6,083,674
_28165
PS417_28170: PS417_28170 - transcriptional regulator, at 6,083,762 to 6,084,400
_28170
PS417_28175: PS417_28175 - GNAT family acetyltransferase, at 6,084,397 to 6,084,843
_28175
PS417_28180: PS417_28180 - GNAT family acetyltransferase, at 6,084,854 to 6,085,525
_28180
Position (kb)
6083
6084
6085 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6082.803 kb on + strand, within PS417_28165 at 6083.072 kb on + strand, within PS417_28165 at 6083.073 kb on - strand, within PS417_28165 at 6083.073 kb on - strand, within PS417_28165 at 6083.073 kb on - strand, within PS417_28165 at 6083.073 kb on - strand, within PS417_28165 at 6083.076 kb on + strand, within PS417_28165 at 6083.421 kb on + strand, within PS417_28165 at 6083.463 kb on + strand, within PS417_28165 at 6083.464 kb on - strand, within PS417_28165 at 6083.464 kb on - strand, within PS417_28165 at 6083.592 kb on + strand at 6083.592 kb on + strand at 6083.593 kb on - strand at 6083.593 kb on - strand at 6083.708 kb on + strand at 6083.708 kb on + strand at 6083.709 kb on - strand at 6083.721 kb on + strand at 6083.731 kb on - strand at 6083.765 kb on + strand at 6083.765 kb on + strand at 6083.765 kb on + strand at 6083.765 kb on + strand at 6083.766 kb on - strand at 6083.766 kb on - strand at 6084.011 kb on + strand, within PS417_28170 at 6084.011 kb on + strand, within PS417_28170 at 6084.012 kb on - strand, within PS417_28170 at 6084.074 kb on + strand, within PS417_28170 at 6084.075 kb on - strand, within PS417_28170 at 6084.108 kb on + strand, within PS417_28170 at 6084.213 kb on - strand, within PS417_28170 at 6084.466 kb on + strand, within PS417_28175 at 6084.466 kb on + strand, within PS417_28175 at 6084.467 kb on - strand, within PS417_28175 at 6084.479 kb on - strand, within PS417_28175 at 6084.547 kb on - strand, within PS417_28175 at 6084.550 kb on + strand, within PS417_28175 at 6084.600 kb on + strand, within PS417_28175 at 6084.600 kb on + strand, within PS417_28175 at 6085.013 kb on + strand, within PS417_28180 at 6085.013 kb on + strand, within PS417_28180 at 6085.014 kb on - strand, within PS417_28180 at 6085.091 kb on + strand, within PS417_28180 at 6085.129 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.242 kb on - strand, within PS417_28180 at 6085.256 kb on + strand, within PS417_28180 at 6085.256 kb on + strand, within PS417_28180 at 6085.257 kb on - strand, within PS417_28180 at 6085.257 kb on - strand, within PS417_28180 at 6085.322 kb on + strand, within PS417_28180 at 6085.322 kb on + strand, within PS417_28180
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 6,082,803 + PS417_28165 0.43 +0.7 6,083,072 + PS417_28165 0.61 +0.3 6,083,073 - PS417_28165 0.61 -1.1 6,083,073 - PS417_28165 0.61 +0.8 6,083,073 - PS417_28165 0.61 -1.3 6,083,073 - PS417_28165 0.61 -1.9 6,083,076 + PS417_28165 0.61 -1.7 6,083,421 + PS417_28165 0.83 +0.6 6,083,463 + PS417_28165 0.86 -0.2 6,083,464 - PS417_28165 0.86 +1.3 6,083,464 - PS417_28165 0.86 +0.2 6,083,592 + +1.2 6,083,592 + +0.2 6,083,593 - -1.5 6,083,593 - -1.8 6,083,708 + -1.7 6,083,708 + +0.3 6,083,709 - -0.2 6,083,721 + -0.1 6,083,731 - -1.9 6,083,765 + -0.6 6,083,765 + -0.0 6,083,765 + +0.8 6,083,765 + +0.1 6,083,766 - -0.8 6,083,766 - +0.0 6,084,011 + PS417_28170 0.39 +1.7 6,084,011 + PS417_28170 0.39 +0.4 6,084,012 - PS417_28170 0.39 +2.2 6,084,074 + PS417_28170 0.49 -0.1 6,084,075 - PS417_28170 0.49 -0.2 6,084,108 + PS417_28170 0.54 -0.1 6,084,213 - PS417_28170 0.71 +1.1 6,084,466 + PS417_28175 0.15 -0.2 6,084,466 + PS417_28175 0.15 -0.2 6,084,467 - PS417_28175 0.16 -0.3 6,084,479 - PS417_28175 0.18 -0.7 6,084,547 - PS417_28175 0.34 +0.8 6,084,550 + PS417_28175 0.34 +1.1 6,084,600 + PS417_28175 0.45 -1.4 6,084,600 + PS417_28175 0.45 -0.0 6,085,013 + PS417_28180 0.24 -1.2 6,085,013 + PS417_28180 0.24 +0.2 6,085,014 - PS417_28180 0.24 +0.3 6,085,091 + PS417_28180 0.35 -0.4 6,085,129 - PS417_28180 0.41 -0.8 6,085,242 - PS417_28180 0.58 +1.7 6,085,242 - PS417_28180 0.58 +1.2 6,085,242 - PS417_28180 0.58 -0.8 6,085,256 + PS417_28180 0.60 -2.7 6,085,256 + PS417_28180 0.60 -0.6 6,085,257 - PS417_28180 0.60 +0.8 6,085,257 - PS417_28180 0.60 +0.3 6,085,322 + PS417_28180 0.70 -1.3 6,085,322 + PS417_28180 0.70 -0.1
Or see this region's nucleotide sequence