Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25670 and PS417_25675 are separated by 70 nucleotides PS417_25675 and PS417_25680 are separated by 63 nucleotides PS417_25680 and PS417_25685 are separated by 46 nucleotides
PS417_25670: PS417_25670 - 2-nitropropane dioxygenase, at 5,544,522 to 5,545,484
_25670
PS417_25675: PS417_25675 - membrane protein, at 5,545,555 to 5,546,511
_25675
PS417_25680: PS417_25680 - short-chain dehydrogenase, at 5,546,575 to 5,547,363
_25680
PS417_25685: PS417_25685 - zinc-binding protein, at 5,547,410 to 5,547,748
_25685
Position (kb) 5545
5546
5547 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5544.825 kb on - strand, within PS417_25670 at 5544.853 kb on + strand, within PS417_25670 at 5544.854 kb on - strand, within PS417_25670 at 5544.862 kb on + strand, within PS417_25670 at 5545.111 kb on - strand, within PS417_25670 at 5545.161 kb on + strand, within PS417_25670 at 5545.357 kb on + strand, within PS417_25670 at 5545.358 kb on - strand, within PS417_25670 at 5545.426 kb on + strand at 5545.426 kb on + strand at 5545.426 kb on + strand at 5545.426 kb on + strand at 5545.522 kb on + strand at 5545.522 kb on + strand at 5545.736 kb on - strand, within PS417_25675 at 5545.904 kb on - strand, within PS417_25675 at 5546.069 kb on + strand, within PS417_25675 at 5546.115 kb on - strand, within PS417_25675 at 5546.164 kb on + strand, within PS417_25675 at 5546.248 kb on + strand, within PS417_25675 at 5546.383 kb on - strand, within PS417_25675 at 5546.395 kb on + strand, within PS417_25675 at 5546.395 kb on + strand, within PS417_25675 at 5546.395 kb on + strand, within PS417_25675 at 5546.492 kb on + strand at 5546.493 kb on - strand at 5547.028 kb on + strand at 5547.028 kb on + strand, within PS417_25680 at 5547.029 kb on - strand, within PS417_25680 at 5547.029 kb on - strand, within PS417_25680 at 5547.032 kb on - strand at 5547.032 kb on - strand, within PS417_25680 at 5547.046 kb on + strand, within PS417_25680 at 5547.046 kb on + strand, within PS417_25680 at 5547.046 kb on + strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.047 kb on - strand, within PS417_25680 at 5547.217 kb on + strand, within PS417_25680 at 5547.217 kb on + strand, within PS417_25680 at 5547.218 kb on - strand, within PS417_25680 at 5547.218 kb on - strand, within PS417_25680 at 5547.349 kb on + strand at 5547.350 kb on - strand at 5547.373 kb on + strand at 5547.374 kb on - strand at 5547.394 kb on + strand at 5547.395 kb on - strand at 5547.447 kb on + strand, within PS417_25685 at 5547.447 kb on + strand, within PS417_25685 at 5547.448 kb on - strand, within PS417_25685 at 5547.465 kb on - strand, within PS417_25685 at 5547.465 kb on - strand, within PS417_25685
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 5,544,825 - PS417_25670 0.31 +0.3 5,544,853 + PS417_25670 0.34 +0.7 5,544,854 - PS417_25670 0.34 -1.0 5,544,862 + PS417_25670 0.35 +0.6 5,545,111 - PS417_25670 0.61 +0.6 5,545,161 + PS417_25670 0.66 +1.6 5,545,357 + PS417_25670 0.87 -0.1 5,545,358 - PS417_25670 0.87 +2.4 5,545,426 + +0.6 5,545,426 + -1.0 5,545,426 + -1.4 5,545,426 + -1.8 5,545,522 + -2.7 5,545,522 + +1.6 5,545,736 - PS417_25675 0.19 -1.8 5,545,904 - PS417_25675 0.36 -2.4 5,546,069 + PS417_25675 0.54 -1.3 5,546,115 - PS417_25675 0.59 +0.4 5,546,164 + PS417_25675 0.64 -1.5 5,546,248 + PS417_25675 0.72 -0.2 5,546,383 - PS417_25675 0.87 -0.1 5,546,395 + PS417_25675 0.88 +0.5 5,546,395 + PS417_25675 0.88 -1.2 5,546,395 + PS417_25675 0.88 +0.3 5,546,492 + +0.4 5,546,493 - +0.2 5,547,028 + +1.1 5,547,028 + PS417_25680 0.57 +0.1 5,547,029 - PS417_25680 0.58 -1.1 5,547,029 - PS417_25680 0.58 +0.4 5,547,032 - +3.6 5,547,032 - PS417_25680 0.58 +0.4 5,547,046 + PS417_25680 0.60 +0.1 5,547,046 + PS417_25680 0.60 +0.2 5,547,046 + PS417_25680 0.60 -0.1 5,547,047 - PS417_25680 0.60 +0.8 5,547,047 - PS417_25680 0.60 -0.1 5,547,047 - PS417_25680 0.60 +1.8 5,547,047 - PS417_25680 0.60 -0.9 5,547,217 + PS417_25680 0.81 +0.8 5,547,217 + PS417_25680 0.81 +0.7 5,547,218 - PS417_25680 0.81 -2.7 5,547,218 - PS417_25680 0.81 +0.3 5,547,349 + -0.7 5,547,350 - -2.0 5,547,373 + +0.3 5,547,374 - -0.4 5,547,394 + +0.2 5,547,395 - +0.1 5,547,447 + PS417_25685 0.11 +0.6 5,547,447 + PS417_25685 0.11 -0.9 5,547,448 - PS417_25685 0.11 +0.9 5,547,465 - PS417_25685 0.16 -1.3 5,547,465 - PS417_25685 0.16 +0.1
Or see this region's nucleotide sequence