Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02330 and PS417_02335 are separated by 55 nucleotides PS417_02335 and PS417_02340 are separated by 101 nucleotides PS417_02340 and PS417_02345 are separated by 23 nucleotides
PS417_02330: PS417_02330 - FAD-dependent oxidoreductase, at 532,496 to 533,671
_02330
PS417_02335: PS417_02335 - multidrug DMT transporter, at 533,727 to 534,062
_02335
PS417_02340: PS417_02340 - LysR family transcriptional regulator, at 534,164 to 535,054
_02340
PS417_02345: PS417_02345 - 3-deoxy-D-manno-octulosonic acid transferase, at 535,078 to 536,355
_02345
Position (kb) 534
535
536 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 533.706 kb on - strand at 533.707 kb on + strand at 533.708 kb on - strand at 533.708 kb on - strand at 533.824 kb on + strand, within PS417_02335 at 533.824 kb on + strand, within PS417_02335 at 533.825 kb on - strand, within PS417_02335 at 534.043 kb on + strand at 534.043 kb on + strand at 534.043 kb on + strand at 534.044 kb on - strand at 534.046 kb on + strand at 534.046 kb on + strand at 534.046 kb on + strand at 534.046 kb on + strand at 534.047 kb on - strand at 534.047 kb on - strand at 534.049 kb on + strand at 534.049 kb on + strand at 534.050 kb on - strand at 534.050 kb on - strand at 534.092 kb on - strand at 534.183 kb on + strand at 534.183 kb on + strand at 534.366 kb on + strand, within PS417_02340 at 534.527 kb on + strand, within PS417_02340 at 534.833 kb on + strand, within PS417_02340 at 534.834 kb on - strand, within PS417_02340 at 534.834 kb on - strand, within PS417_02340 at 534.889 kb on + strand, within PS417_02340
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 533,706 - -1.5 533,707 + -0.2 533,708 - -0.1 533,708 - -1.6 533,824 + PS417_02335 0.29 -0.2 533,824 + PS417_02335 0.29 -1.7 533,825 - PS417_02335 0.29 +0.1 534,043 + +1.1 534,043 + -0.1 534,043 + +0.2 534,044 - +0.0 534,046 + -0.1 534,046 + -0.3 534,046 + -0.3 534,046 + +0.5 534,047 - +1.0 534,047 - +0.1 534,049 + -0.9 534,049 + +1.2 534,050 - -0.7 534,050 - +0.5 534,092 - -0.5 534,183 + +0.7 534,183 + -0.5 534,366 + PS417_02340 0.23 -1.1 534,527 + PS417_02340 0.41 -4.3 534,833 + PS417_02340 0.75 +0.1 534,834 - PS417_02340 0.75 -1.8 534,834 - PS417_02340 0.75 +0.4 534,889 + PS417_02340 0.81 +0.3
Or see this region's nucleotide sequence