Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23310 and PS417_23315 are separated by 209 nucleotides PS417_23315 and PS417_23320 are separated by 195 nucleotides PS417_23320 and PS417_23325 overlap by 4 nucleotides
PS417_23310: PS417_23310 - glyoxalase, at 5,045,587 to 5,046,018
_23310
PS417_23315: PS417_23315 - transcriptional regulator, at 5,046,228 to 5,046,608
_23315
PS417_23320: PS417_23320 - heme oxygenase, at 5,046,804 to 5,047,418
_23320
PS417_23325: PS417_23325 - histidine kinase, at 5,047,415 to 5,049,658
_23325
Position (kb) 5046
5047
5048 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5045.855 kb on + strand, within PS417_23310 at 5045.856 kb on - strand, within PS417_23310 at 5045.856 kb on - strand, within PS417_23310 at 5045.856 kb on - strand, within PS417_23310 at 5046.018 kb on + strand at 5046.019 kb on - strand at 5046.019 kb on - strand at 5046.019 kb on - strand at 5046.136 kb on + strand at 5046.137 kb on - strand at 5046.137 kb on - strand at 5046.137 kb on - strand at 5046.189 kb on + strand at 5046.189 kb on + strand at 5046.200 kb on + strand at 5046.200 kb on + strand at 5046.200 kb on + strand at 5046.200 kb on + strand at 5046.200 kb on + strand at 5046.201 kb on - strand at 5046.351 kb on + strand, within PS417_23315 at 5046.489 kb on - strand, within PS417_23315 at 5046.719 kb on - strand at 5046.723 kb on + strand at 5046.724 kb on - strand at 5046.741 kb on - strand at 5046.741 kb on - strand at 5046.933 kb on + strand, within PS417_23320 at 5046.934 kb on - strand, within PS417_23320 at 5046.955 kb on - strand, within PS417_23320 at 5046.955 kb on - strand, within PS417_23320 at 5046.958 kb on - strand, within PS417_23320 at 5046.958 kb on - strand, within PS417_23320 at 5046.958 kb on - strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.161 kb on + strand, within PS417_23320 at 5047.259 kb on + strand, within PS417_23320 at 5047.263 kb on + strand, within PS417_23320 at 5047.264 kb on - strand, within PS417_23320 at 5047.264 kb on - strand, within PS417_23320 at 5047.408 kb on + strand at 5047.408 kb on + strand at 5047.409 kb on - strand at 5047.409 kb on - strand at 5047.660 kb on + strand, within PS417_23325 at 5047.660 kb on + strand, within PS417_23325 at 5047.660 kb on + strand, within PS417_23325 at 5047.661 kb on - strand, within PS417_23325 at 5047.661 kb on - strand, within PS417_23325 at 5047.919 kb on + strand, within PS417_23325 at 5048.181 kb on - strand, within PS417_23325 at 5048.181 kb on - strand, within PS417_23325
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 5,045,855 + PS417_23310 0.62 -0.9 5,045,856 - PS417_23310 0.62 -2.0 5,045,856 - PS417_23310 0.62 +0.1 5,045,856 - PS417_23310 0.62 -0.1 5,046,018 + +0.2 5,046,019 - -2.1 5,046,019 - -1.8 5,046,019 - -0.6 5,046,136 + +0.1 5,046,137 - +0.2 5,046,137 - +0.0 5,046,137 - -0.1 5,046,189 + +0.3 5,046,189 + +0.2 5,046,200 + -0.5 5,046,200 + +2.3 5,046,200 + +1.4 5,046,200 + -0.3 5,046,200 + +0.7 5,046,201 - +0.6 5,046,351 + PS417_23315 0.32 +1.3 5,046,489 - PS417_23315 0.69 -1.2 5,046,719 - +0.4 5,046,723 + -1.0 5,046,724 - +0.1 5,046,741 - -1.2 5,046,741 - +1.2 5,046,933 + PS417_23320 0.21 +0.2 5,046,934 - PS417_23320 0.21 -0.1 5,046,955 - PS417_23320 0.25 -1.0 5,046,955 - PS417_23320 0.25 +1.3 5,046,958 - PS417_23320 0.25 -1.2 5,046,958 - PS417_23320 0.25 -0.2 5,046,958 - PS417_23320 0.25 -1.1 5,047,161 + PS417_23320 0.58 +0.0 5,047,161 + PS417_23320 0.58 -0.0 5,047,161 + PS417_23320 0.58 +0.3 5,047,161 + PS417_23320 0.58 -0.6 5,047,161 + PS417_23320 0.58 +0.2 5,047,161 + PS417_23320 0.58 +0.3 5,047,161 + PS417_23320 0.58 -0.2 5,047,259 + PS417_23320 0.74 -0.5 5,047,263 + PS417_23320 0.75 +0.5 5,047,264 - PS417_23320 0.75 +0.3 5,047,264 - PS417_23320 0.75 +0.2 5,047,408 + -1.1 5,047,408 + +1.3 5,047,409 - +0.2 5,047,409 - +0.8 5,047,660 + PS417_23325 0.11 +0.3 5,047,660 + PS417_23325 0.11 -1.0 5,047,660 + PS417_23325 0.11 +1.2 5,047,661 - PS417_23325 0.11 +1.2 5,047,661 - PS417_23325 0.11 +2.1 5,047,919 + PS417_23325 0.22 -0.6 5,048,181 - PS417_23325 0.34 -3.5 5,048,181 - PS417_23325 0.34 -1.1
Or see this region's nucleotide sequence