Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22165 and PS417_22170 are separated by 98 nucleotides PS417_22170 and PS417_22175 are separated by 5 nucleotides PS417_22175 and PS417_22180 are separated by 178 nucleotides PS417_22180 and PS417_22185 are separated by 139 nucleotides
PS417_22165: PS417_22165 - LysR family transcriptional regulator, at 4,802,344 to 4,803,237
_22165
PS417_22170: PS417_22170 - acetyltransferase, at 4,803,336 to 4,803,737
_22170
PS417_22175: PS417_22175 - amino acid transporter LysE, at 4,803,743 to 4,804,342
_22175
PS417_22180: PS417_22180 - superoxide dismutase, at 4,804,521 to 4,805,117
_22180
PS417_22185: PS417_22185 - hypothetical protein, at 4,805,257 to 4,805,442
_22185
Position (kb)
4803
4804
4805 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4803.226 kb on - strand at 4803.280 kb on - strand at 4803.321 kb on + strand at 4803.322 kb on - strand at 4803.323 kb on + strand at 4803.324 kb on - strand at 4803.324 kb on - strand at 4803.402 kb on + strand, within PS417_22170 at 4803.402 kb on + strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.654 kb on + strand at 4803.706 kb on + strand at 4803.706 kb on + strand at 4803.707 kb on - strand at 4803.736 kb on - strand at 4803.742 kb on + strand at 4803.743 kb on - strand at 4803.820 kb on - strand, within PS417_22175 at 4804.178 kb on + strand, within PS417_22175 at 4804.414 kb on + strand at 4805.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 4,803,226 - -0.1 4,803,280 - -0.2 4,803,321 + -0.7 4,803,322 - -0.2 4,803,323 + +1.6 4,803,324 - -1.0 4,803,324 - -0.4 4,803,402 + PS417_22170 0.16 -0.6 4,803,402 + PS417_22170 0.16 +0.0 4,803,403 - PS417_22170 0.17 -1.8 4,803,403 - PS417_22170 0.17 -0.0 4,803,654 + +1.6 4,803,706 + -1.6 4,803,706 + -0.0 4,803,707 - +0.9 4,803,736 - +0.7 4,803,742 + +0.3 4,803,743 - +3.9 4,803,820 - PS417_22175 0.13 -1.3 4,804,178 + PS417_22175 0.72 -0.2 4,804,414 + -2.0 4,805,116 - -0.0
Or see this region's nucleotide sequence