Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01900 and PS417_01905 are separated by 149 nucleotides PS417_01905 and PS417_01910 are separated by 249 nucleotides PS417_01910 and PS417_01915 are separated by 148 nucleotides
PS417_01900: PS417_01900 - poly(3-hydroxyalkanoate) depolymerase, at 420,224 to 421,069
_01900
PS417_01905: PS417_01905 - poly(R)-hydroxyalkanoic acid synthase, at 421,219 to 422,898
_01905
PS417_01910: PS417_01910 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 423,148 to 423,525
_01910
PS417_01915: PS417_01915 - ATP-dependent protease ATP-binding subunit HslU, at 423,674 to 425,011
_01915
Position (kb) 421
422
423 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 420.225 kb on + strand at 420.259 kb on + strand at 420.260 kb on - strand at 420.499 kb on - strand, within PS417_01900 at 420.622 kb on - strand, within PS417_01900 at 420.673 kb on + strand, within PS417_01900 at 420.793 kb on - strand, within PS417_01900 at 420.802 kb on - strand, within PS417_01900 at 421.046 kb on - strand at 421.094 kb on + strand at 421.094 kb on + strand at 421.094 kb on + strand at 421.094 kb on + strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.272 kb on + strand at 421.275 kb on + strand at 421.276 kb on - strand at 421.279 kb on + strand at 421.279 kb on + strand at 421.280 kb on - strand at 421.280 kb on - strand at 421.282 kb on + strand at 421.481 kb on - strand, within PS417_01905 at 421.481 kb on - strand, within PS417_01905 at 421.481 kb on - strand, within PS417_01905 at 421.568 kb on + strand at 421.568 kb on + strand, within PS417_01905 at 421.751 kb on + strand, within PS417_01905 at 421.755 kb on + strand, within PS417_01905 at 421.755 kb on + strand, within PS417_01905 at 421.756 kb on - strand, within PS417_01905 at 421.756 kb on - strand, within PS417_01905 at 421.767 kb on - strand, within PS417_01905 at 421.974 kb on - strand, within PS417_01905 at 422.090 kb on + strand, within PS417_01905 at 422.090 kb on + strand, within PS417_01905 at 422.209 kb on + strand, within PS417_01905 at 422.210 kb on - strand, within PS417_01905 at 422.210 kb on - strand, within PS417_01905 at 422.214 kb on - strand, within PS417_01905 at 422.283 kb on - strand, within PS417_01905 at 422.609 kb on + strand, within PS417_01905 at 422.610 kb on - strand, within PS417_01905 at 422.622 kb on - strand, within PS417_01905 at 422.630 kb on + strand, within PS417_01905 at 422.892 kb on + strand at 422.893 kb on - strand at 422.901 kb on - strand at 423.599 kb on + strand at 423.676 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 420,225 + +0.4 420,259 + +0.1 420,260 - -0.7 420,499 - PS417_01900 0.33 +0.1 420,622 - PS417_01900 0.47 -0.2 420,673 + PS417_01900 0.53 -1.7 420,793 - PS417_01900 0.67 -2.4 420,802 - PS417_01900 0.68 -0.3 421,046 - -0.9 421,094 + +1.0 421,094 + -0.4 421,094 + +0.8 421,094 + -0.1 421,095 - +0.3 421,095 - -0.9 421,095 - -0.4 421,095 - +0.7 421,095 - -0.2 421,095 - +0.2 421,095 - -0.7 421,235 + -0.6 421,235 + -1.1 421,235 + -0.5 421,235 + +3.3 421,235 + +3.7 421,236 - -0.7 421,236 - -2.1 421,236 - -1.4 421,236 - -0.1 421,236 - -0.3 421,236 - +0.3 421,272 + +0.3 421,275 + -3.4 421,276 - +0.1 421,279 + -1.3 421,279 + +0.4 421,280 - -0.8 421,280 - -0.4 421,282 + +0.2 421,481 - PS417_01905 0.16 +0.5 421,481 - PS417_01905 0.16 -0.3 421,481 - PS417_01905 0.16 -0.9 421,568 + -0.3 421,568 + PS417_01905 0.21 +4.5 421,751 + PS417_01905 0.32 +2.3 421,755 + PS417_01905 0.32 +0.9 421,755 + PS417_01905 0.32 -2.5 421,756 - PS417_01905 0.32 +1.5 421,756 - PS417_01905 0.32 +1.4 421,767 - PS417_01905 0.33 -1.3 421,974 - PS417_01905 0.45 +0.4 422,090 + PS417_01905 0.52 +0.3 422,090 + PS417_01905 0.52 -0.8 422,209 + PS417_01905 0.59 -0.6 422,210 - PS417_01905 0.59 +0.9 422,210 - PS417_01905 0.59 +0.7 422,214 - PS417_01905 0.59 +0.5 422,283 - PS417_01905 0.63 +1.5 422,609 + PS417_01905 0.83 +3.4 422,610 - PS417_01905 0.83 -0.3 422,622 - PS417_01905 0.84 -0.5 422,630 + PS417_01905 0.84 +0.1 422,892 + -0.2 422,893 - +0.6 422,901 - +1.4 423,599 + -0.0 423,676 - +0.3
Or see this region's nucleotide sequence