Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01895 and PS417_01900 are separated by 88 nucleotides PS417_01900 and PS417_01905 are separated by 149 nucleotides
PS417_01895: PS417_01895 - poly(R)-hydroxyalkanoic acid synthase, at 418,453 to 420,135
_01895
PS417_01900: PS417_01900 - poly(3-hydroxyalkanoate) depolymerase, at 420,224 to 421,069
_01900
PS417_01905: PS417_01905 - poly(R)-hydroxyalkanoic acid synthase, at 421,219 to 422,898
_01905
Position (kb) 420
421
422 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 419.327 kb on + strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.356 kb on + strand at 419.356 kb on + strand, within PS417_01895 at 419.448 kb on + strand, within PS417_01895 at 419.448 kb on + strand, within PS417_01895 at 419.448 kb on + strand, within PS417_01895 at 419.450 kb on + strand, within PS417_01895 at 419.451 kb on - strand, within PS417_01895 at 419.451 kb on - strand, within PS417_01895 at 419.453 kb on + strand, within PS417_01895 at 419.454 kb on - strand, within PS417_01895 at 419.454 kb on - strand, within PS417_01895 at 419.454 kb on - strand, within PS417_01895 at 419.495 kb on + strand, within PS417_01895 at 419.519 kb on + strand, within PS417_01895 at 419.519 kb on + strand, within PS417_01895 at 419.520 kb on - strand, within PS417_01895 at 419.846 kb on + strand, within PS417_01895 at 419.846 kb on + strand, within PS417_01895 at 419.847 kb on - strand, within PS417_01895 at 420.188 kb on + strand at 420.225 kb on + strand at 420.259 kb on + strand at 420.260 kb on - strand at 420.499 kb on - strand, within PS417_01900 at 420.622 kb on - strand, within PS417_01900 at 420.673 kb on + strand, within PS417_01900 at 420.793 kb on - strand, within PS417_01900 at 420.802 kb on - strand, within PS417_01900 at 421.046 kb on - strand at 421.094 kb on + strand at 421.094 kb on + strand at 421.094 kb on + strand at 421.094 kb on + strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.095 kb on - strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.235 kb on + strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.236 kb on - strand at 421.272 kb on + strand at 421.275 kb on + strand at 421.276 kb on - strand at 421.279 kb on + strand at 421.279 kb on + strand at 421.280 kb on - strand at 421.280 kb on - strand at 421.282 kb on + strand at 421.481 kb on - strand, within PS417_01905 at 421.481 kb on - strand, within PS417_01905 at 421.481 kb on - strand, within PS417_01905 at 421.568 kb on + strand at 421.568 kb on + strand, within PS417_01905 at 421.751 kb on + strand, within PS417_01905 at 421.755 kb on + strand, within PS417_01905 at 421.755 kb on + strand, within PS417_01905 at 421.756 kb on - strand, within PS417_01905 at 421.756 kb on - strand, within PS417_01905 at 421.767 kb on - strand, within PS417_01905 at 421.974 kb on - strand, within PS417_01905
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 419,327 + PS417_01895 0.52 +0.1 419,328 - PS417_01895 0.52 +1.0 419,328 - PS417_01895 0.52 +1.7 419,328 - PS417_01895 0.52 -1.7 419,328 - PS417_01895 0.52 +0.9 419,356 + +1.3 419,356 + PS417_01895 0.54 -0.5 419,448 + PS417_01895 0.59 -0.7 419,448 + PS417_01895 0.59 -0.1 419,448 + PS417_01895 0.59 -2.2 419,450 + PS417_01895 0.59 -0.3 419,451 - PS417_01895 0.59 +0.1 419,451 - PS417_01895 0.59 +0.1 419,453 + PS417_01895 0.59 -0.7 419,454 - PS417_01895 0.59 +0.1 419,454 - PS417_01895 0.59 +0.1 419,454 - PS417_01895 0.59 -0.0 419,495 + PS417_01895 0.62 +0.5 419,519 + PS417_01895 0.63 -1.4 419,519 + PS417_01895 0.63 -2.5 419,520 - PS417_01895 0.63 -0.6 419,846 + PS417_01895 0.83 -0.3 419,846 + PS417_01895 0.83 -0.1 419,847 - PS417_01895 0.83 +0.1 420,188 + +0.0 420,225 + +0.4 420,259 + +0.1 420,260 - -0.7 420,499 - PS417_01900 0.33 +0.1 420,622 - PS417_01900 0.47 -0.2 420,673 + PS417_01900 0.53 -1.7 420,793 - PS417_01900 0.67 -2.4 420,802 - PS417_01900 0.68 -0.3 421,046 - -0.9 421,094 + +1.0 421,094 + -0.4 421,094 + +0.8 421,094 + -0.1 421,095 - +0.3 421,095 - -0.9 421,095 - -0.4 421,095 - +0.7 421,095 - -0.2 421,095 - +0.2 421,095 - -0.7 421,235 + -0.6 421,235 + -1.1 421,235 + -0.5 421,235 + +3.3 421,235 + +3.7 421,236 - -0.7 421,236 - -2.1 421,236 - -1.4 421,236 - -0.1 421,236 - -0.3 421,236 - +0.3 421,272 + +0.3 421,275 + -3.4 421,276 - +0.1 421,279 + -1.3 421,279 + +0.4 421,280 - -0.8 421,280 - -0.4 421,282 + +0.2 421,481 - PS417_01905 0.16 +0.5 421,481 - PS417_01905 0.16 -0.3 421,481 - PS417_01905 0.16 -0.9 421,568 + -0.3 421,568 + PS417_01905 0.21 +4.5 421,751 + PS417_01905 0.32 +2.3 421,755 + PS417_01905 0.32 +0.9 421,755 + PS417_01905 0.32 -2.5 421,756 - PS417_01905 0.32 +1.5 421,756 - PS417_01905 0.32 +1.4 421,767 - PS417_01905 0.33 -1.3 421,974 - PS417_01905 0.45 +0.4
Or see this region's nucleotide sequence