Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15520 and PS417_15525 are separated by 120 nucleotides PS417_15525 and PS417_15530 overlap by 10 nucleotides PS417_15530 and PS417_15535 are separated by 36 nucleotides
PS417_15520: PS417_15520 - hypothetical protein, at 3,398,235 to 3,398,645
_15520
PS417_15525: PS417_15525 - AraC family transcriptional regulator, at 3,398,766 to 3,399,632
_15525
PS417_15530: PS417_15530 - ribonuclease, at 3,399,623 to 3,400,252
_15530
PS417_15535: PS417_15535 - oxidoreductase, at 3,400,289 to 3,401,269
_15535
Position (kb)
3399
3400
3401 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3398.690 kb on + strand at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3398.913 kb on + strand, within PS417_15525 at 3399.039 kb on + strand, within PS417_15525 at 3399.040 kb on - strand, within PS417_15525 at 3399.040 kb on - strand, within PS417_15525 at 3399.156 kb on + strand, within PS417_15525 at 3399.156 kb on + strand, within PS417_15525 at 3399.156 kb on + strand, within PS417_15525 at 3399.157 kb on - strand, within PS417_15525 at 3399.157 kb on - strand, within PS417_15525 at 3399.157 kb on - strand, within PS417_15525 at 3399.646 kb on + strand at 3399.825 kb on + strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.928 kb on + strand, within PS417_15530 at 3399.929 kb on - strand, within PS417_15530 at 3400.053 kb on + strand, within PS417_15530 at 3400.065 kb on - strand, within PS417_15530 at 3400.076 kb on + strand, within PS417_15530 at 3400.077 kb on - strand, within PS417_15530 at 3400.077 kb on - strand, within PS417_15530 at 3400.286 kb on + strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.290 kb on + strand at 3400.316 kb on + strand at 3400.317 kb on - strand at 3400.692 kb on + strand, within PS417_15535 at 3400.692 kb on + strand, within PS417_15535 at 3400.734 kb on + strand, within PS417_15535 at 3401.026 kb on - strand, within PS417_15535 at 3401.059 kb on - strand at 3401.107 kb on - strand, within PS417_15535 at 3401.199 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 3,398,690 + +0.2 3,398,913 + PS417_15525 0.17 +0.9 3,398,913 + PS417_15525 0.17 -1.3 3,398,913 + PS417_15525 0.17 +0.4 3,398,913 + PS417_15525 0.17 +1.8 3,398,913 + PS417_15525 0.17 -0.5 3,399,039 + PS417_15525 0.31 +0.1 3,399,040 - PS417_15525 0.32 +0.3 3,399,040 - PS417_15525 0.32 -0.9 3,399,156 + PS417_15525 0.45 +0.5 3,399,156 + PS417_15525 0.45 +0.1 3,399,156 + PS417_15525 0.45 -1.8 3,399,157 - PS417_15525 0.45 -0.4 3,399,157 - PS417_15525 0.45 +2.3 3,399,157 - PS417_15525 0.45 +0.5 3,399,646 + +0.5 3,399,825 + PS417_15530 0.32 +1.0 3,399,826 - PS417_15530 0.32 -0.1 3,399,826 - PS417_15530 0.32 -1.2 3,399,826 - PS417_15530 0.32 -0.7 3,399,928 + PS417_15530 0.48 -0.5 3,399,929 - PS417_15530 0.49 -0.3 3,400,053 + PS417_15530 0.68 -0.6 3,400,065 - PS417_15530 0.70 -3.8 3,400,076 + PS417_15530 0.72 +0.5 3,400,077 - PS417_15530 0.72 +0.0 3,400,077 - PS417_15530 0.72 -1.1 3,400,286 + -0.8 3,400,287 - -1.2 3,400,287 - +0.0 3,400,287 - -0.2 3,400,287 - -0.0 3,400,290 + -0.4 3,400,316 + -0.8 3,400,317 - -1.4 3,400,692 + PS417_15535 0.41 +0.2 3,400,692 + PS417_15535 0.41 +0.3 3,400,734 + PS417_15535 0.45 -1.2 3,401,026 - PS417_15535 0.75 +2.2 3,401,059 - -0.3 3,401,107 - PS417_15535 0.83 -0.7 3,401,199 + -0.6
Or see this region's nucleotide sequence