Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15085 and PS417_15090 are separated by 5 nucleotides PS417_15090 and PS417_15095 overlap by 4 nucleotides PS417_15095 and PS417_15100 overlap by 8 nucleotides
PS417_15085: PS417_15085 - ATPase AAA, at 3,299,291 to 3,300,250
_15085
PS417_15090: PS417_15090 - hypothetical protein, at 3,300,256 to 3,301,188
_15090
PS417_15095: PS417_15095 - hypothetical protein, at 3,301,185 to 3,301,679
_15095
PS417_15100: PS417_15100 - BatB protein, at 3,301,672 to 3,302,760
_15100
Position (kb)
3300
3301
3302 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3299.335 kb on + strand at 3299.335 kb on + strand at 3299.336 kb on - strand at 3299.402 kb on - strand, within PS417_15085 at 3299.537 kb on + strand, within PS417_15085 at 3299.690 kb on + strand, within PS417_15085 at 3299.691 kb on - strand, within PS417_15085 at 3299.753 kb on + strand, within PS417_15085 at 3299.833 kb on + strand, within PS417_15085 at 3299.939 kb on + strand, within PS417_15085 at 3299.940 kb on - strand, within PS417_15085 at 3299.940 kb on - strand, within PS417_15085 at 3299.963 kb on + strand, within PS417_15085 at 3299.963 kb on + strand, within PS417_15085 at 3300.425 kb on - strand, within PS417_15090 at 3301.039 kb on + strand, within PS417_15090 at 3301.039 kb on + strand, within PS417_15090 at 3301.492 kb on - strand, within PS417_15095 at 3301.580 kb on - strand, within PS417_15095 at 3301.586 kb on - strand, within PS417_15095 at 3301.780 kb on + strand at 3302.108 kb on - strand, within PS417_15100
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 3,299,335 + -1.0 3,299,335 + +0.9 3,299,336 - +1.3 3,299,402 - PS417_15085 0.12 -0.3 3,299,537 + PS417_15085 0.26 -1.9 3,299,690 + PS417_15085 0.42 +2.5 3,299,691 - PS417_15085 0.42 +1.3 3,299,753 + PS417_15085 0.48 -0.9 3,299,833 + PS417_15085 0.56 -1.5 3,299,939 + PS417_15085 0.68 +0.3 3,299,940 - PS417_15085 0.68 +0.3 3,299,940 - PS417_15085 0.68 +1.3 3,299,963 + PS417_15085 0.70 -1.3 3,299,963 + PS417_15085 0.70 -0.7 3,300,425 - PS417_15090 0.18 +0.3 3,301,039 + PS417_15090 0.84 +0.3 3,301,039 + PS417_15090 0.84 -1.4 3,301,492 - PS417_15095 0.62 +0.2 3,301,580 - PS417_15095 0.80 +1.3 3,301,586 - PS417_15095 0.81 -0.1 3,301,780 + -0.7 3,302,108 - PS417_15100 0.40 +1.3
Or see this region's nucleotide sequence