Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14315 and PS417_14320 are separated by 3 nucleotides PS417_14320 and PS417_14325 are separated by 20 nucleotides
PS417_14315: PS417_14315 - 2,5-dioxovalerate dehydrogenase, at 3,133,196 to 3,134,767
_14315
PS417_14320: PS417_14320 - FAD-dependent oxidoreductase, at 3,134,771 to 3,136,009
_14320
PS417_14325: PS417_14325 - AraC family transcriptional regulator, at 3,136,030 to 3,137,037
_14325
Position (kb)
3134
3135
3136
3137 Strain fitness (log2 ratio)
-2
-1
0
1 at 3134.012 kb on + strand, within PS417_14315 at 3134.013 kb on - strand, within PS417_14315 at 3134.013 kb on - strand, within PS417_14315 at 3134.620 kb on + strand at 3134.620 kb on + strand at 3134.620 kb on + strand at 3134.620 kb on + strand at 3134.791 kb on - strand at 3134.898 kb on - strand, within PS417_14320 at 3134.898 kb on - strand, within PS417_14320 at 3135.077 kb on + strand, within PS417_14320 at 3135.171 kb on + strand, within PS417_14320 at 3135.172 kb on - strand at 3135.372 kb on - strand, within PS417_14320 at 3136.031 kb on + strand at 3136.080 kb on + strand at 3136.081 kb on - strand at 3136.081 kb on - strand at 3136.082 kb on + strand at 3136.083 kb on - strand at 3136.083 kb on - strand at 3136.200 kb on - strand at 3136.200 kb on - strand, within PS417_14325 at 3136.467 kb on + strand, within PS417_14325 at 3136.845 kb on + strand, within PS417_14325 at 3136.989 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 3,134,012 + PS417_14315 0.52 +0.7 3,134,013 - PS417_14315 0.52 +0.4 3,134,013 - PS417_14315 0.52 -1.0 3,134,620 + +0.3 3,134,620 + +0.2 3,134,620 + +0.3 3,134,620 + -0.7 3,134,791 - +0.3 3,134,898 - PS417_14320 0.10 +0.3 3,134,898 - PS417_14320 0.10 +0.7 3,135,077 + PS417_14320 0.25 +0.1 3,135,171 + PS417_14320 0.32 +1.5 3,135,172 - +0.3 3,135,372 - PS417_14320 0.49 -0.2 3,136,031 + -0.4 3,136,080 + -1.3 3,136,081 - -0.1 3,136,081 - -1.6 3,136,082 + -0.2 3,136,083 - +0.5 3,136,083 - +0.9 3,136,200 - -1.9 3,136,200 - PS417_14325 0.17 -0.8 3,136,467 + PS417_14325 0.43 -0.2 3,136,845 + PS417_14325 0.81 -0.1 3,136,989 - +1.7
Or see this region's nucleotide sequence