Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13045 and PS417_13050 are separated by 51 nucleotides PS417_13050 and PS417_13055 overlap by 4 nucleotides PS417_13055 and PS417_13060 are separated by 104 nucleotides
PS417_13045: PS417_13045 - gamma-glutamyltranspeptidase, at 2,850,868 to 2,852,592
_13045
PS417_13050: PS417_13050 - N-acetylglucosamine-6-sulfatase, at 2,852,644 to 2,853,162
_13050
PS417_13055: PS417_13055 - aminotransferase, at 2,853,159 to 2,853,722
_13055
PS417_13060: PS417_13060 - chemotaxis protein, at 2,853,827 to 2,855,461
_13060
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-1
0
1 at 2851.676 kb on - strand, within PS417_13045 at 2851.822 kb on + strand, within PS417_13045 at 2851.822 kb on + strand, within PS417_13045 at 2851.822 kb on + strand, within PS417_13045 at 2851.841 kb on - strand, within PS417_13045 at 2851.841 kb on - strand, within PS417_13045 at 2852.042 kb on - strand, within PS417_13045 at 2852.042 kb on - strand, within PS417_13045 at 2852.042 kb on - strand, within PS417_13045 at 2852.149 kb on + strand, within PS417_13045 at 2852.149 kb on + strand, within PS417_13045 at 2852.150 kb on - strand, within PS417_13045 at 2852.150 kb on - strand, within PS417_13045 at 2852.150 kb on - strand, within PS417_13045 at 2852.938 kb on - strand, within PS417_13050 at 2853.027 kb on + strand, within PS417_13050 at 2853.056 kb on + strand, within PS417_13050 at 2853.124 kb on - strand at 2853.124 kb on - strand at 2853.259 kb on + strand, within PS417_13055 at 2853.539 kb on - strand, within PS417_13055 at 2853.614 kb on - strand, within PS417_13055
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,851,676 - PS417_13045 0.47 -0.9 2,851,822 + PS417_13045 0.55 -0.7 2,851,822 + PS417_13045 0.55 -0.2 2,851,822 + PS417_13045 0.55 +0.8 2,851,841 - PS417_13045 0.56 -0.5 2,851,841 - PS417_13045 0.56 +0.0 2,852,042 - PS417_13045 0.68 +0.8 2,852,042 - PS417_13045 0.68 -0.0 2,852,042 - PS417_13045 0.68 -0.3 2,852,149 + PS417_13045 0.74 -1.3 2,852,149 + PS417_13045 0.74 -0.5 2,852,150 - PS417_13045 0.74 -0.7 2,852,150 - PS417_13045 0.74 +1.6 2,852,150 - PS417_13045 0.74 +1.5 2,852,938 - PS417_13050 0.57 -1.5 2,853,027 + PS417_13050 0.74 -0.5 2,853,056 + PS417_13050 0.79 -0.9 2,853,124 - -1.0 2,853,124 - -1.3 2,853,259 + PS417_13055 0.18 -1.4 2,853,539 - PS417_13055 0.67 +1.3 2,853,614 - PS417_13055 0.81 -0.1
Or see this region's nucleotide sequence