Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01290 and PS417_01295 overlap by 4 nucleotides PS417_01295 and PS417_01300 are separated by 126 nucleotides PS417_01300 and PS417_01305 are separated by 26 nucleotides
PS417_01290: PS417_01290 - ADP-ribose diphosphatase, at 280,235 to 280,801
_01290
PS417_01295: PS417_01295 - adenosine-3'(2'),5'-bisphosphate nucleotidase, at 280,798 to 281,634
_01295
PS417_01300: PS417_01300 - thioesterase, at 281,761 to 282,216
_01300
PS417_01305: PS417_01305 - Fis family transcriptional regulator, at 282,243 to 283,619
_01305
Position (kb) 281
282
283 Strain fitness (log2 ratio) -2
-1
0
1
2 at 281.192 kb on - strand, within PS417_01295 at 281.192 kb on - strand, within PS417_01295 at 281.577 kb on - strand at 281.645 kb on - strand at 281.778 kb on - strand at 281.778 kb on - strand at 281.867 kb on + strand, within PS417_01300 at 281.868 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 282.291 kb on + strand at 282.291 kb on + strand at 282.291 kb on + strand at 282.428 kb on + strand at 282.542 kb on + strand at 282.542 kb on + strand, within PS417_01305 at 282.701 kb on - strand, within PS417_01305 at 282.703 kb on + strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 281,192 - PS417_01295 0.47 +2.2 281,192 - PS417_01295 0.47 -1.2 281,577 - -1.7 281,645 - -1.5 281,778 - -0.3 281,778 - -0.7 281,867 + PS417_01300 0.23 -1.2 281,868 - PS417_01300 0.23 -1.3 281,977 - PS417_01300 0.47 -1.1 281,977 - PS417_01300 0.47 -1.5 281,977 - PS417_01300 0.47 -0.5 281,977 - PS417_01300 0.47 -0.4 281,977 - PS417_01300 0.47 -0.4 282,291 + -0.4 282,291 + +1.1 282,291 + -0.0 282,428 + +0.6 282,542 + +1.6 282,542 + PS417_01305 0.22 -0.9 282,701 - PS417_01305 0.33 -0.3 282,703 + PS417_01305 0.33 -2.2 282,704 - PS417_01305 0.33 -0.2 282,704 - PS417_01305 0.33 -0.1 282,704 - PS417_01305 0.33 +0.5
Or see this region's nucleotide sequence