Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12905 and PS417_12910 are separated by 286 nucleotides PS417_12910 and PS417_12915 are separated by 70 nucleotides PS417_12915 and PS417_12920 overlap by 13 nucleotides
PS417_12905: PS417_12905 - ribonucleotide-diphosphate reductase subunit beta, at 2,823,069 to 2,824,319
_12905
PS417_12910: PS417_12910 - alanine racemase, at 2,824,606 to 2,825,703
_12910
PS417_12915: PS417_12915 - DNA-binding protein, at 2,825,774 to 2,826,400
_12915
PS417_12920: PS417_12920 - endoribonuclease, at 2,826,388 to 2,826,768
_12920
Position (kb)
2824
2825
2826 Strain fitness (log2 ratio)
-2
-1
0
1 at 2824.325 kb on + strand at 2824.388 kb on - strand at 2824.388 kb on - strand at 2824.390 kb on + strand at 2824.390 kb on + strand at 2824.391 kb on - strand at 2824.403 kb on - strand at 2824.423 kb on + strand at 2824.434 kb on - strand at 2824.443 kb on + strand at 2824.444 kb on - strand at 2824.444 kb on - strand at 2824.448 kb on - strand at 2824.448 kb on - strand at 2824.475 kb on + strand at 2824.475 kb on + strand at 2824.475 kb on + strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.476 kb on - strand at 2824.477 kb on + strand at 2824.477 kb on + strand at 2824.477 kb on + strand at 2824.477 kb on + strand at 2824.478 kb on - strand at 2824.478 kb on - strand at 2824.604 kb on + strand at 2824.605 kb on - strand at 2824.630 kb on + strand at 2824.631 kb on - strand at 2824.636 kb on + strand at 2824.636 kb on + strand at 2824.636 kb on + strand at 2824.664 kb on + strand at 2824.987 kb on + strand, within PS417_12910 at 2824.988 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand, within PS417_12910 at 2825.009 kb on - strand at 2825.762 kb on - strand at 2825.783 kb on - strand at 2825.783 kb on - strand at 2826.083 kb on + strand, within PS417_12915 at 2826.084 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.289 kb on - strand, within PS417_12915 at 2826.384 kb on - strand at 2826.389 kb on + strand at 2826.389 kb on + strand at 2826.398 kb on + strand at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.539 kb on + strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.540 kb on - strand, within PS417_12920 at 2826.598 kb on + strand, within PS417_12920 at 2826.689 kb on + strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920 at 2826.690 kb on - strand, within PS417_12920
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,824,325 + -0.1 2,824,388 - +1.1 2,824,388 - -1.9 2,824,390 + +0.2 2,824,390 + +0.3 2,824,391 - +0.5 2,824,403 - -0.3 2,824,423 + +1.7 2,824,434 - -1.4 2,824,443 + -2.3 2,824,444 - +0.2 2,824,444 - -0.5 2,824,448 - -0.5 2,824,448 - -0.6 2,824,475 + +0.4 2,824,475 + -0.2 2,824,475 + -0.4 2,824,476 - +1.3 2,824,476 - -0.3 2,824,476 - -2.4 2,824,476 - -0.3 2,824,476 - +0.0 2,824,477 + -0.5 2,824,477 + +0.8 2,824,477 + -0.7 2,824,477 + -0.3 2,824,478 - -1.7 2,824,478 - -1.7 2,824,604 + -0.4 2,824,605 - -0.7 2,824,630 + +1.5 2,824,631 - +0.3 2,824,636 + -1.1 2,824,636 + -0.9 2,824,636 + -0.5 2,824,664 + +1.3 2,824,987 + PS417_12910 0.35 +0.3 2,824,988 - PS417_12910 0.35 -1.4 2,825,009 - PS417_12910 0.37 -2.4 2,825,009 - PS417_12910 0.37 -0.0 2,825,009 - PS417_12910 0.37 -1.5 2,825,009 - +0.3 2,825,762 - +1.0 2,825,783 - +0.3 2,825,783 - -0.7 2,826,083 + PS417_12915 0.49 -0.0 2,826,084 - PS417_12915 0.49 -1.7 2,826,289 - PS417_12915 0.82 -1.1 2,826,289 - PS417_12915 0.82 -2.2 2,826,384 - +0.6 2,826,389 + +0.1 2,826,389 + +0.3 2,826,398 + +0.8 2,826,539 + PS417_12920 0.40 +0.0 2,826,539 + PS417_12920 0.40 -2.5 2,826,539 + PS417_12920 0.40 +0.0 2,826,540 - PS417_12920 0.40 -0.3 2,826,540 - PS417_12920 0.40 +0.5 2,826,540 - PS417_12920 0.40 -0.4 2,826,598 + PS417_12920 0.55 +0.4 2,826,689 + PS417_12920 0.79 -1.5 2,826,690 - PS417_12920 0.79 -0.2 2,826,690 - PS417_12920 0.79 +0.2 2,826,690 - PS417_12920 0.79 -0.4 2,826,690 - PS417_12920 0.79 -0.8
Or see this region's nucleotide sequence