Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12240 and PS417_12245 are separated by 20 nucleotides PS417_12245 and PS417_12250 overlap by 11 nucleotides
PS417_12240: PS417_12240 - lysine decarboxylase, at 2,659,927 to 2,662,182
_12240
PS417_12245: PS417_12245 - GNAT family acetyltransferase, at 2,662,203 to 2,662,757
_12245
PS417_12250: PS417_12250 - LysR family transcriptional regulator, at 2,662,747 to 2,663,667
_12250
Position (kb)
2662
2663 Strain fitness (log2 ratio)
-2
-1
0
1 at 2661.204 kb on + strand, within PS417_12240 at 2661.492 kb on - strand, within PS417_12240 at 2661.628 kb on + strand, within PS417_12240 at 2661.641 kb on + strand, within PS417_12240 at 2661.641 kb on + strand, within PS417_12240 at 2661.826 kb on + strand, within PS417_12240 at 2661.866 kb on - strand, within PS417_12240 at 2661.908 kb on - strand, within PS417_12240 at 2662.157 kb on - strand at 2662.157 kb on - strand at 2662.190 kb on + strand at 2662.190 kb on + strand at 2662.190 kb on + strand at 2662.216 kb on - strand at 2662.261 kb on - strand, within PS417_12245 at 2662.351 kb on + strand, within PS417_12245 at 2662.464 kb on - strand, within PS417_12245 at 2662.464 kb on - strand, within PS417_12245 at 2662.647 kb on + strand, within PS417_12245 at 2662.662 kb on + strand at 2662.755 kb on + strand at 2662.868 kb on + strand, within PS417_12250 at 2662.997 kb on + strand, within PS417_12250 at 2662.998 kb on - strand, within PS417_12250 at 2663.129 kb on + strand, within PS417_12250 at 2663.130 kb on - strand, within PS417_12250 at 2663.130 kb on - strand, within PS417_12250 at 2663.333 kb on - strand, within PS417_12250 at 2663.573 kb on - strand, within PS417_12250 at 2663.573 kb on - strand, within PS417_12250 at 2663.679 kb on - strand at 2663.679 kb on - strand at 2663.703 kb on + strand at 2663.703 kb on + strand at 2663.703 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,661,204 + PS417_12240 0.57 -0.5 2,661,492 - PS417_12240 0.69 -1.0 2,661,628 + PS417_12240 0.75 -0.2 2,661,641 + PS417_12240 0.76 -1.5 2,661,641 + PS417_12240 0.76 +1.1 2,661,826 + PS417_12240 0.84 -0.1 2,661,866 - PS417_12240 0.86 -0.1 2,661,908 - PS417_12240 0.88 +0.2 2,662,157 - +0.5 2,662,157 - -0.3 2,662,190 + -0.1 2,662,190 + -0.3 2,662,190 + +0.3 2,662,216 - +0.6 2,662,261 - PS417_12245 0.10 -0.6 2,662,351 + PS417_12245 0.27 -1.5 2,662,464 - PS417_12245 0.47 -0.2 2,662,464 - PS417_12245 0.47 -1.8 2,662,647 + PS417_12245 0.80 -1.9 2,662,662 + -0.3 2,662,755 + -0.6 2,662,868 + PS417_12250 0.13 +0.3 2,662,997 + PS417_12250 0.27 -0.0 2,662,998 - PS417_12250 0.27 +0.7 2,663,129 + PS417_12250 0.41 -0.9 2,663,130 - PS417_12250 0.42 +0.7 2,663,130 - PS417_12250 0.42 -0.8 2,663,333 - PS417_12250 0.64 -2.7 2,663,573 - PS417_12250 0.90 +0.3 2,663,573 - PS417_12250 0.90 -0.6 2,663,679 - -0.7 2,663,679 - -0.7 2,663,703 + +1.5 2,663,703 + -0.7 2,663,703 + -0.1
Or see this region's nucleotide sequence