Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12125 and PS417_12130 are separated by 10 nucleotides PS417_12130 and PS417_12135 are separated by 63 nucleotides PS417_12135 and PS417_12140 are separated by 43 nucleotides
PS417_12125: PS417_12125 - D-glucosaminate dehydratase (EC 4.3.1.9) (from data) , at 2,630,658 to 2,631,866
_12125
PS417_12130: PS417_12130 - ketodeoxygluconokinase, at 2,631,877 to 2,632,806
_12130
PS417_12135: PS417_12135 - peptidylprolyl isomerase, at 2,632,870 to 2,633,145
_12135
PS417_12140: PS417_12140 - histidine kinase, at 2,633,189 to 2,635,684
_12140
Position (kb)
2631
2632
2633 Strain fitness (log2 ratio)
-1
0
1 at 2631.109 kb on - strand, within PS417_12125 at 2631.216 kb on + strand, within PS417_12125 at 2631.418 kb on + strand, within PS417_12125 at 2631.435 kb on + strand at 2631.436 kb on - strand, within PS417_12125 at 2631.459 kb on + strand, within PS417_12125 at 2631.459 kb on + strand, within PS417_12125 at 2631.459 kb on + strand, within PS417_12125 at 2631.460 kb on - strand, within PS417_12125 at 2631.460 kb on - strand, within PS417_12125 at 2631.798 kb on - strand at 2631.995 kb on - strand, within PS417_12130 at 2631.995 kb on - strand, within PS417_12130 at 2632.034 kb on - strand, within PS417_12130 at 2632.147 kb on + strand, within PS417_12130 at 2632.148 kb on - strand, within PS417_12130 at 2632.148 kb on - strand, within PS417_12130 at 2632.279 kb on + strand, within PS417_12130 at 2632.280 kb on - strand, within PS417_12130 at 2632.435 kb on + strand, within PS417_12130 at 2632.786 kb on + strand at 2633.077 kb on + strand, within PS417_12135 at 2633.178 kb on + strand at 2633.178 kb on + strand at 2633.397 kb on - strand at 2633.710 kb on - strand, within PS417_12140
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,631,109 - PS417_12125 0.37 -1.4 2,631,216 + PS417_12125 0.46 +0.7 2,631,418 + PS417_12125 0.63 -0.3 2,631,435 + +1.3 2,631,436 - PS417_12125 0.64 -1.3 2,631,459 + PS417_12125 0.66 +0.4 2,631,459 + PS417_12125 0.66 -0.2 2,631,459 + PS417_12125 0.66 -0.3 2,631,460 - PS417_12125 0.66 -0.0 2,631,460 - PS417_12125 0.66 -0.3 2,631,798 - -0.1 2,631,995 - PS417_12130 0.13 -0.9 2,631,995 - PS417_12130 0.13 +0.1 2,632,034 - PS417_12130 0.17 -0.9 2,632,147 + PS417_12130 0.29 -0.4 2,632,148 - PS417_12130 0.29 -1.7 2,632,148 - PS417_12130 0.29 -1.6 2,632,279 + PS417_12130 0.43 +0.5 2,632,280 - PS417_12130 0.43 -0.5 2,632,435 + PS417_12130 0.60 -0.3 2,632,786 + -0.2 2,633,077 + PS417_12135 0.75 -0.0 2,633,178 + -0.8 2,633,178 + +0.4 2,633,397 - +0.7 2,633,710 - PS417_12140 0.21 -0.5
Or see this region's nucleotide sequence