Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11115 and PS417_11120 are separated by 74 nucleotides PS417_11120 and PS417_11125 are separated by 180 nucleotides PS417_11125 and PS417_11130 are separated by 256 nucleotides
PS417_11115: PS417_11115 - peroxidase, at 2,382,546 to 2,383,502
_11115
PS417_11120: PS417_11120 - UDP pyrophosphate phosphatase, at 2,383,577 to 2,384,446
_11120
PS417_11125: PS417_11125 - hypothetical protein, at 2,384,627 to 2,385,133
_11125
PS417_11130: PS417_11130 - peptide ABC transporter substrate-binding protein, at 2,385,390 to 2,386,946
_11130
Position (kb)
2383
2384
2385 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2382.969 kb on + strand, within PS417_11115 at 2383.086 kb on + strand, within PS417_11115 at 2383.087 kb on - strand, within PS417_11115 at 2383.105 kb on - strand, within PS417_11115 at 2383.112 kb on + strand, within PS417_11115 at 2383.113 kb on - strand, within PS417_11115 at 2383.323 kb on + strand, within PS417_11115 at 2383.323 kb on + strand, within PS417_11115 at 2383.404 kb on + strand, within PS417_11115 at 2383.404 kb on + strand, within PS417_11115 at 2384.166 kb on + strand, within PS417_11120 at 2384.167 kb on - strand, within PS417_11120 at 2384.181 kb on - strand, within PS417_11120 at 2384.312 kb on + strand, within PS417_11120 at 2384.316 kb on - strand, within PS417_11120 at 2384.449 kb on + strand at 2384.449 kb on + strand at 2384.450 kb on - strand at 2384.526 kb on + strand at 2384.549 kb on - strand at 2384.700 kb on - strand, within PS417_11125 at 2384.708 kb on + strand, within PS417_11125 at 2384.834 kb on + strand, within PS417_11125 at 2384.834 kb on + strand, within PS417_11125 at 2384.835 kb on - strand, within PS417_11125 at 2385.000 kb on - strand, within PS417_11125 at 2385.000 kb on - strand, within PS417_11125 at 2385.326 kb on + strand at 2385.327 kb on - strand at 2385.327 kb on - strand at 2385.327 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,382,969 + PS417_11115 0.44 -0.7 2,383,086 + PS417_11115 0.56 +1.5 2,383,087 - PS417_11115 0.57 -0.5 2,383,105 - PS417_11115 0.58 +0.1 2,383,112 + PS417_11115 0.59 +0.0 2,383,113 - PS417_11115 0.59 -2.0 2,383,323 + PS417_11115 0.81 -0.3 2,383,323 + PS417_11115 0.81 +2.6 2,383,404 + PS417_11115 0.90 -1.1 2,383,404 + PS417_11115 0.90 +0.3 2,384,166 + PS417_11120 0.68 +0.0 2,384,167 - PS417_11120 0.68 -0.2 2,384,181 - PS417_11120 0.69 -1.5 2,384,312 + PS417_11120 0.84 -0.9 2,384,316 - PS417_11120 0.85 -1.3 2,384,449 + +1.3 2,384,449 + -0.4 2,384,450 - +0.0 2,384,526 + +1.3 2,384,549 - -0.6 2,384,700 - PS417_11125 0.14 -0.3 2,384,708 + PS417_11125 0.16 +0.1 2,384,834 + PS417_11125 0.41 -2.0 2,384,834 + PS417_11125 0.41 -1.3 2,384,835 - PS417_11125 0.41 -0.2 2,385,000 - PS417_11125 0.74 +1.5 2,385,000 - PS417_11125 0.74 -0.5 2,385,326 + +0.2 2,385,327 - -0.3 2,385,327 - -0.1 2,385,327 - +0.2
Or see this region's nucleotide sequence