Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10660 and PS417_10665 are separated by 134 nucleotides PS417_10665 and PS417_10670 overlap by 4 nucleotides
PS417_10660: PS417_10660 - histidine kinase, at 2,275,191 to 2,278,805
_10660
PS417_10665: PS417_10665 - hypothetical protein, at 2,278,940 to 2,279,365
_10665
PS417_10670: PS417_10670 - 2,5-diketo-D-gluconic acid reductase, at 2,279,362 to 2,280,180
_10670
Position (kb)
2278
2279
2280 Strain fitness (log2 ratio)
-1
0
1
2 at 2278.041 kb on + strand, within PS417_10660 at 2278.124 kb on - strand, within PS417_10660 at 2278.256 kb on + strand, within PS417_10660 at 2278.256 kb on + strand, within PS417_10660 at 2278.256 kb on + strand, within PS417_10660 at 2278.257 kb on - strand, within PS417_10660 at 2278.257 kb on - strand, within PS417_10660 at 2278.257 kb on - strand, within PS417_10660 at 2278.257 kb on - strand, within PS417_10660 at 2278.257 kb on - strand, within PS417_10660 at 2278.257 kb on - strand, within PS417_10660 at 2278.257 kb on - strand, within PS417_10660 at 2278.272 kb on - strand, within PS417_10660 at 2278.484 kb on + strand at 2278.846 kb on - strand at 2278.860 kb on + strand at 2278.884 kb on - strand at 2278.884 kb on - strand at 2279.174 kb on + strand, within PS417_10665 at 2279.175 kb on - strand, within PS417_10665 at 2279.633 kb on + strand, within PS417_10670 at 2279.633 kb on + strand, within PS417_10670 at 2279.633 kb on + strand, within PS417_10670 at 2279.634 kb on - strand, within PS417_10670 at 2279.634 kb on - strand, within PS417_10670 at 2279.897 kb on + strand, within PS417_10670 at 2280.050 kb on + strand, within PS417_10670 at 2280.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,278,041 + PS417_10660 0.79 -0.1 2,278,124 - PS417_10660 0.81 -0.2 2,278,256 + PS417_10660 0.85 +0.5 2,278,256 + PS417_10660 0.85 +0.1 2,278,256 + PS417_10660 0.85 -0.6 2,278,257 - PS417_10660 0.85 +0.6 2,278,257 - PS417_10660 0.85 +1.6 2,278,257 - PS417_10660 0.85 -0.5 2,278,257 - PS417_10660 0.85 -0.2 2,278,257 - PS417_10660 0.85 -0.1 2,278,257 - PS417_10660 0.85 -0.3 2,278,257 - PS417_10660 0.85 +2.1 2,278,272 - PS417_10660 0.85 +0.8 2,278,484 + +1.6 2,278,846 - +0.1 2,278,860 + +0.2 2,278,884 - -0.4 2,278,884 - -1.1 2,279,174 + PS417_10665 0.55 -0.7 2,279,175 - PS417_10665 0.55 -1.5 2,279,633 + PS417_10670 0.33 +0.4 2,279,633 + PS417_10670 0.33 +1.4 2,279,633 + PS417_10670 0.33 -0.6 2,279,634 - PS417_10670 0.33 -0.6 2,279,634 - PS417_10670 0.33 -0.4 2,279,897 + PS417_10670 0.65 -1.2 2,280,050 + PS417_10670 0.84 -0.4 2,280,316 - +0.3
Or see this region's nucleotide sequence