Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10400 and PS417_10405 overlap by 4 nucleotides PS417_10405 and PS417_10410 are separated by 91 nucleotides PS417_10410 and PS417_10415 are separated by 225 nucleotides
PS417_10400: PS417_10400 - membrane protein, at 2,224,145 to 2,225,212
_10400
PS417_10405: PS417_10405 - filamentous hemagglutinin, at 2,225,209 to 2,225,379
_10405
PS417_10410: PS417_10410 - hypothetical protein, at 2,225,471 to 2,225,665
_10410
PS417_10415: PS417_10415 - hypothetical protein, at 2,225,891 to 2,226,265
_10415
Position (kb)
2225
2226 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2224.490 kb on + strand, within PS417_10400 at 2224.652 kb on + strand, within PS417_10400 at 2224.653 kb on - strand, within PS417_10400 at 2224.662 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.941 kb on - strand, within PS417_10400 at 2225.259 kb on + strand at 2225.259 kb on + strand, within PS417_10405 at 2225.410 kb on + strand at 2225.428 kb on - strand at 2225.759 kb on + strand at 2225.760 kb on - strand at 2225.760 kb on - strand at 2225.949 kb on + strand, within PS417_10415 at 2225.950 kb on - strand, within PS417_10415 at 2225.973 kb on + strand, within PS417_10415 at 2225.974 kb on - strand, within PS417_10415 at 2225.974 kb on - strand at 2225.974 kb on - strand, within PS417_10415 at 2225.994 kb on + strand, within PS417_10415 at 2225.995 kb on - strand, within PS417_10415 at 2226.012 kb on + strand, within PS417_10415 at 2226.012 kb on + strand, within PS417_10415 at 2226.013 kb on - strand, within PS417_10415 at 2226.013 kb on - strand, within PS417_10415 at 2226.037 kb on - strand, within PS417_10415 at 2226.078 kb on + strand, within PS417_10415 at 2226.078 kb on + strand, within PS417_10415 at 2226.079 kb on - strand, within PS417_10415 at 2226.079 kb on - strand, within PS417_10415 at 2226.097 kb on - strand, within PS417_10415 at 2226.250 kb on + strand at 2226.250 kb on + strand at 2226.304 kb on - strand at 2226.304 kb on - strand at 2226.313 kb on + strand at 2226.313 kb on + strand at 2226.314 kb on - strand at 2226.333 kb on + strand at 2226.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,224,490 + PS417_10400 0.32 +1.4 2,224,652 + PS417_10400 0.47 +1.2 2,224,653 - PS417_10400 0.48 -3.1 2,224,662 - PS417_10400 0.48 -0.1 2,224,697 - PS417_10400 0.52 -0.6 2,224,697 - PS417_10400 0.52 -0.9 2,224,697 - PS417_10400 0.52 -2.2 2,224,941 - PS417_10400 0.75 +0.4 2,225,259 + -0.6 2,225,259 + PS417_10405 0.29 -0.9 2,225,410 + +0.7 2,225,428 - +0.2 2,225,759 + +0.6 2,225,760 - +1.2 2,225,760 - -1.5 2,225,949 + PS417_10415 0.15 -1.5 2,225,950 - PS417_10415 0.16 -0.2 2,225,973 + PS417_10415 0.22 -1.0 2,225,974 - PS417_10415 0.22 +1.2 2,225,974 - +1.4 2,225,974 - PS417_10415 0.22 -1.8 2,225,994 + PS417_10415 0.27 -0.7 2,225,995 - PS417_10415 0.28 +1.4 2,226,012 + PS417_10415 0.32 +1.4 2,226,012 + PS417_10415 0.32 +0.1 2,226,013 - PS417_10415 0.33 +1.2 2,226,013 - PS417_10415 0.33 +0.2 2,226,037 - PS417_10415 0.39 +0.6 2,226,078 + PS417_10415 0.50 -1.2 2,226,078 + PS417_10415 0.50 +2.6 2,226,079 - PS417_10415 0.50 -0.4 2,226,079 - PS417_10415 0.50 -0.9 2,226,097 - PS417_10415 0.55 +1.0 2,226,250 + +1.0 2,226,250 + +1.4 2,226,304 - +1.0 2,226,304 - -0.8 2,226,313 + -0.3 2,226,313 + +0.4 2,226,314 - -1.3 2,226,333 + -1.4 2,226,344 - +0.4
Or see this region's nucleotide sequence