Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10315 and PS417_10320 are separated by 1 nucleotides PS417_10320 and PS417_10325 are separated by 84 nucleotides PS417_10325 and PS417_10330 overlap by 1 nucleotides
PS417_10315: PS417_10315 - cytochrome C biogenesis protein, at 2,205,287 to 2,206,495
_10315
PS417_10320: PS417_10320 - hypothetical protein, at 2,206,497 to 2,207,117
_10320
PS417_10325: PS417_10325 - chemotaxis protein CheY, at 2,207,202 to 2,207,573
_10325
PS417_10330: PS417_10330 - histidine kinase, at 2,207,573 to 2,209,135
_10330
Position (kb)
2206
2207
2208 Strain fitness (log2 ratio)
-2
-1
0
1 at 2205.924 kb on + strand, within PS417_10315 at 2206.001 kb on + strand, within PS417_10315 at 2206.002 kb on - strand, within PS417_10315 at 2206.151 kb on + strand, within PS417_10315 at 2206.151 kb on + strand, within PS417_10315 at 2206.151 kb on + strand, within PS417_10315 at 2206.152 kb on - strand, within PS417_10315 at 2206.152 kb on - strand, within PS417_10315 at 2206.185 kb on - strand, within PS417_10315 at 2206.209 kb on - strand, within PS417_10315 at 2206.337 kb on + strand, within PS417_10315 at 2206.338 kb on - strand, within PS417_10315 at 2206.640 kb on + strand at 2206.640 kb on + strand, within PS417_10320 at 2206.743 kb on - strand, within PS417_10320 at 2207.094 kb on + strand at 2207.095 kb on - strand at 2207.169 kb on - strand at 2207.182 kb on + strand at 2207.182 kb on + strand at 2207.183 kb on - strand at 2207.183 kb on - strand at 2207.745 kb on + strand, within PS417_10330 at 2207.745 kb on + strand, within PS417_10330 at 2207.815 kb on - strand, within PS417_10330 at 2207.878 kb on + strand, within PS417_10330 at 2207.988 kb on - strand, within PS417_10330
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,205,924 + PS417_10315 0.53 +1.0 2,206,001 + PS417_10315 0.59 +0.1 2,206,002 - PS417_10315 0.59 -1.4 2,206,151 + PS417_10315 0.71 -0.4 2,206,151 + PS417_10315 0.71 +0.4 2,206,151 + PS417_10315 0.71 -2.6 2,206,152 - PS417_10315 0.72 +0.9 2,206,152 - PS417_10315 0.72 +0.2 2,206,185 - PS417_10315 0.74 +0.1 2,206,209 - PS417_10315 0.76 -0.0 2,206,337 + PS417_10315 0.87 -0.3 2,206,338 - PS417_10315 0.87 +0.2 2,206,640 + -0.2 2,206,640 + PS417_10320 0.23 -1.7 2,206,743 - PS417_10320 0.40 -0.0 2,207,094 + +0.0 2,207,095 - +0.1 2,207,169 - -0.1 2,207,182 + +0.8 2,207,182 + +1.4 2,207,183 - -0.6 2,207,183 - -0.6 2,207,745 + PS417_10330 0.11 +1.1 2,207,745 + PS417_10330 0.11 +1.0 2,207,815 - PS417_10330 0.15 -0.0 2,207,878 + PS417_10330 0.20 +0.1 2,207,988 - PS417_10330 0.27 +0.8
Or see this region's nucleotide sequence