Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09670 and PS417_09675 overlap by 67 nucleotides PS417_09675 and PS417_09680 are separated by 74 nucleotides PS417_09680 and PS417_09685 are separated by 48 nucleotides
PS417_09670: PS417_09670 - AraC family transcriptional regulator, at 2,096,969 to 2,097,973
_09670
PS417_09675: PS417_09675 - alpha/beta hydrolase, at 2,097,907 to 2,098,821
_09675
PS417_09680: PS417_09680 - hydrolase, at 2,098,896 to 2,099,369
_09680
PS417_09685: PS417_09685 - glucoamylase, at 2,099,418 to 2,101,244
_09685
Position (kb)
2097
2098
2099 Strain fitness (log2 ratio)
-2
-1
0
1 at 2096.917 kb on - strand at 2096.924 kb on + strand at 2096.925 kb on - strand at 2096.939 kb on - strand at 2097.190 kb on + strand, within PS417_09670 at 2097.978 kb on + strand at 2097.978 kb on + strand at 2097.978 kb on + strand at 2097.979 kb on - strand at 2098.185 kb on - strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.244 kb on + strand, within PS417_09675 at 2098.361 kb on + strand, within PS417_09675 at 2098.427 kb on + strand, within PS417_09675 at 2098.427 kb on + strand, within PS417_09675 at 2098.483 kb on - strand, within PS417_09675 at 2098.648 kb on + strand, within PS417_09675 at 2098.649 kb on - strand, within PS417_09675 at 2098.695 kb on - strand, within PS417_09675 at 2098.766 kb on + strand at 2099.163 kb on - strand, within PS417_09680 at 2099.358 kb on + strand at 2099.359 kb on - strand at 2099.359 kb on - strand at 2099.385 kb on - strand at 2099.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 2,096,917 - +0.2 2,096,924 + -0.2 2,096,925 - +0.3 2,096,939 - -1.2 2,097,190 + PS417_09670 0.22 -0.4 2,097,978 + +0.4 2,097,978 + -1.6 2,097,978 + -0.3 2,097,979 - -1.4 2,098,185 - PS417_09675 0.30 -1.3 2,098,244 + PS417_09675 0.37 -1.0 2,098,244 + PS417_09675 0.37 -1.8 2,098,244 + PS417_09675 0.37 -0.5 2,098,244 + PS417_09675 0.37 -0.3 2,098,361 + PS417_09675 0.50 -0.1 2,098,427 + PS417_09675 0.57 -1.5 2,098,427 + PS417_09675 0.57 +0.4 2,098,483 - PS417_09675 0.63 +0.4 2,098,648 + PS417_09675 0.81 -1.2 2,098,649 - PS417_09675 0.81 -1.8 2,098,695 - PS417_09675 0.86 -0.6 2,098,766 + -0.4 2,099,163 - PS417_09680 0.56 +0.2 2,099,358 + -1.6 2,099,359 - -0.6 2,099,359 - -0.7 2,099,385 - -0.0 2,099,511 - +0.6
Or see this region's nucleotide sequence