Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08215 and PS417_08220 overlap by 4 nucleotides PS417_08220 and PS417_08225 overlap by 1 nucleotides PS417_08225 and PS417_08230 are separated by 3 nucleotides
PS417_08215: PS417_08215 - flippase, at 1,799,291 to 1,800,601
_08215
PS417_08220: PS417_08220 - epimerase, at 1,800,598 to 1,801,530
_08220
PS417_08225: PS417_08225 - acetolactate synthase, at 1,801,530 to 1,803,245
_08225
PS417_08230: PS417_08230 - lipopolysaccharide biosynthesis protein RfbH, at 1,803,249 to 1,804,562
_08230
Position (kb)
1801
1802
1803
1804 Strain fitness (log2 ratio)
-2
-1
0
1 at 1800.702 kb on - strand, within PS417_08220 at 1800.836 kb on - strand at 1800.938 kb on - strand at 1802.276 kb on - strand, within PS417_08225 at 1802.477 kb on - strand, within PS417_08225 at 1802.517 kb on - strand, within PS417_08225 at 1802.521 kb on - strand, within PS417_08225 at 1802.910 kb on - strand, within PS417_08225 at 1802.933 kb on - strand, within PS417_08225 at 1802.933 kb on - strand, within PS417_08225 at 1803.188 kb on - strand at 1803.325 kb on - strand at 1803.407 kb on - strand, within PS417_08230 at 1803.436 kb on - strand, within PS417_08230 at 1803.725 kb on - strand, within PS417_08230
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 1,800,702 - PS417_08220 0.11 +0.5 1,800,836 - +0.9 1,800,938 - +1.5 1,802,276 - PS417_08225 0.43 -0.3 1,802,477 - PS417_08225 0.55 -0.8 1,802,517 - PS417_08225 0.58 -2.1 1,802,521 - PS417_08225 0.58 -0.0 1,802,910 - PS417_08225 0.80 -0.9 1,802,933 - PS417_08225 0.82 +0.3 1,802,933 - PS417_08225 0.82 -1.8 1,803,188 - +0.5 1,803,325 - -0.9 1,803,407 - PS417_08230 0.12 +0.1 1,803,436 - PS417_08230 0.14 -0.5 1,803,725 - PS417_08230 0.36 -1.1
Or see this region's nucleotide sequence