Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06390 and PS417_06395 are separated by 57 nucleotides PS417_06395 and PS417_06400 are separated by 13 nucleotides PS417_06400 and PS417_06405 are separated by 26 nucleotides PS417_06405 and PS417_06410 overlap by 4 nucleotides
PS417_06390: PS417_06390 - DEAD/DEAH box helicase, at 1,415,550 to 1,418,030
_06390
PS417_06395: PS417_06395 - amino acid transporter, at 1,418,088 to 1,418,852
_06395
PS417_06400: PS417_06400 - succinylglutamate desuccinylase, at 1,418,866 to 1,419,984
_06400
PS417_06405: PS417_06405 - amino acid ABC transporter permease, at 1,420,011 to 1,420,721
_06405
PS417_06410: PS417_06410 - amino acid ABC transporter permease, at 1,420,718 to 1,421,446
_06410
Position (kb)
1418
1419
1420 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1417.888 kb on + strand at 1417.888 kb on + strand at 1417.889 kb on - strand at 1417.889 kb on - strand at 1417.954 kb on - strand at 1418.459 kb on - strand, within PS417_06395 at 1418.543 kb on + strand, within PS417_06395 at 1418.543 kb on + strand, within PS417_06395 at 1418.544 kb on - strand, within PS417_06395 at 1418.848 kb on + strand at 1418.848 kb on + strand at 1418.848 kb on + strand at 1418.848 kb on + strand at 1418.878 kb on + strand at 1418.879 kb on - strand at 1419.054 kb on - strand, within PS417_06400 at 1419.076 kb on + strand, within PS417_06400 at 1419.194 kb on + strand, within PS417_06400 at 1419.404 kb on + strand, within PS417_06400 at 1419.404 kb on + strand, within PS417_06400 at 1419.405 kb on - strand, within PS417_06400 at 1419.707 kb on + strand, within PS417_06400 at 1419.884 kb on + strand at 1420.130 kb on + strand, within PS417_06405 at 1420.130 kb on + strand, within PS417_06405 at 1420.230 kb on - strand, within PS417_06405 at 1420.234 kb on + strand, within PS417_06405 at 1420.234 kb on + strand, within PS417_06405 at 1420.235 kb on - strand, within PS417_06405 at 1420.235 kb on - strand, within PS417_06405 at 1420.237 kb on + strand, within PS417_06405 at 1420.276 kb on + strand, within PS417_06405 at 1420.277 kb on - strand, within PS417_06405 at 1420.277 kb on - strand, within PS417_06405 at 1420.474 kb on + strand, within PS417_06405 at 1420.474 kb on + strand, within PS417_06405 at 1420.474 kb on + strand, within PS417_06405 at 1420.487 kb on - strand, within PS417_06405 at 1420.487 kb on - strand, within PS417_06405 at 1420.507 kb on + strand, within PS417_06405 at 1420.507 kb on + strand, within PS417_06405 at 1420.531 kb on + strand, within PS417_06405 at 1420.531 kb on + strand, within PS417_06405 at 1420.532 kb on - strand, within PS417_06405 at 1420.537 kb on + strand, within PS417_06405 at 1420.537 kb on + strand, within PS417_06405 at 1420.557 kb on + strand, within PS417_06405 at 1420.680 kb on - strand at 1420.681 kb on + strand at 1420.682 kb on - strand at 1420.682 kb on - strand at 1420.700 kb on - strand at 1420.750 kb on - strand at 1420.804 kb on - strand at 1420.842 kb on + strand, within PS417_06410 at 1420.842 kb on + strand, within PS417_06410
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 1,417,888 + +1.3 1,417,888 + -0.2 1,417,889 - +0.2 1,417,889 - -1.9 1,417,954 - -0.2 1,418,459 - PS417_06395 0.48 -1.3 1,418,543 + PS417_06395 0.59 -0.2 1,418,543 + PS417_06395 0.59 -2.2 1,418,544 - PS417_06395 0.60 +2.9 1,418,848 + +0.6 1,418,848 + -0.5 1,418,848 + +0.2 1,418,848 + -0.5 1,418,878 + +0.2 1,418,879 - +1.4 1,419,054 - PS417_06400 0.17 -2.7 1,419,076 + PS417_06400 0.19 -1.1 1,419,194 + PS417_06400 0.29 +0.2 1,419,404 + PS417_06400 0.48 -0.3 1,419,404 + PS417_06400 0.48 -0.8 1,419,405 - PS417_06400 0.48 -0.7 1,419,707 + PS417_06400 0.75 -2.2 1,419,884 + -0.7 1,420,130 + PS417_06405 0.17 -0.5 1,420,130 + PS417_06405 0.17 -0.2 1,420,230 - PS417_06405 0.31 -0.5 1,420,234 + PS417_06405 0.31 +0.3 1,420,234 + PS417_06405 0.31 -0.4 1,420,235 - PS417_06405 0.32 -0.1 1,420,235 - PS417_06405 0.32 +0.8 1,420,237 + PS417_06405 0.32 +0.2 1,420,276 + PS417_06405 0.37 -0.3 1,420,277 - PS417_06405 0.37 +0.4 1,420,277 - PS417_06405 0.37 +0.4 1,420,474 + PS417_06405 0.65 -0.2 1,420,474 + PS417_06405 0.65 -0.4 1,420,474 + PS417_06405 0.65 +0.6 1,420,487 - PS417_06405 0.67 -0.6 1,420,487 - PS417_06405 0.67 -0.2 1,420,507 + PS417_06405 0.70 -2.1 1,420,507 + PS417_06405 0.70 +0.7 1,420,531 + PS417_06405 0.73 -0.2 1,420,531 + PS417_06405 0.73 +0.3 1,420,532 - PS417_06405 0.73 -0.7 1,420,537 + PS417_06405 0.74 -1.0 1,420,537 + PS417_06405 0.74 +2.5 1,420,557 + PS417_06405 0.77 -0.1 1,420,680 - +2.5 1,420,681 + -0.1 1,420,682 - +0.4 1,420,682 - -0.4 1,420,700 - +0.9 1,420,750 - +1.1 1,420,804 - -1.3 1,420,842 + PS417_06410 0.17 -2.1 1,420,842 + PS417_06410 0.17 -0.8
Or see this region's nucleotide sequence