Experiment: P. simiae ORA MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06000 and PS417_06005 are separated by 83 nucleotides PS417_06005 and PS417_06010 are separated by 106 nucleotides PS417_06010 and PS417_06015 are separated by 146 nucleotides
PS417_06000: PS417_06000 - peptide chain release factor 2, at 1,333,967 to 1,334,848
_06000
PS417_06005: PS417_06005 - lysyl-tRNA synthetase, at 1,334,932 to 1,336,431
_06005
PS417_06010: PS417_06010 - TetR family transcriptional regulator, at 1,336,538 to 1,337,254
_06010
PS417_06015: PS417_06015 - hypothetical protein, at 1,337,401 to 1,337,949
_06015
Position (kb)
1334
1335
1336
1337 Strain fitness (log2 ratio)
-1
0
1 at 1334.846 kb on + strand at 1334.854 kb on + strand at 1334.854 kb on + strand at 1334.854 kb on + strand at 1334.854 kb on + strand at 1336.459 kb on - strand at 1336.459 kb on - strand at 1336.459 kb on - strand at 1336.520 kb on + strand at 1336.520 kb on + strand at 1336.521 kb on - strand at 1336.760 kb on + strand, within PS417_06010 at 1336.760 kb on + strand, within PS417_06010 at 1336.880 kb on + strand, within PS417_06010 at 1336.946 kb on + strand, within PS417_06010 at 1336.946 kb on + strand, within PS417_06010 at 1336.946 kb on + strand, within PS417_06010 at 1336.947 kb on - strand, within PS417_06010 at 1337.160 kb on - strand, within PS417_06010 at 1337.225 kb on + strand at 1337.274 kb on + strand at 1337.274 kb on + strand at 1337.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 1 remove 1,334,846 + +0.5 1,334,854 + -0.7 1,334,854 + -0.2 1,334,854 + +1.5 1,334,854 + +1.7 1,336,459 - +0.6 1,336,459 - -0.6 1,336,459 - -0.4 1,336,520 + +0.3 1,336,520 + +1.2 1,336,521 - +0.6 1,336,760 + PS417_06010 0.31 -0.6 1,336,760 + PS417_06010 0.31 +0.1 1,336,880 + PS417_06010 0.48 +0.2 1,336,946 + PS417_06010 0.57 +0.3 1,336,946 + PS417_06010 0.57 -1.2 1,336,946 + PS417_06010 0.57 -0.3 1,336,947 - PS417_06010 0.57 +1.2 1,337,160 - PS417_06010 0.87 -0.5 1,337,225 + -0.0 1,337,274 + +1.5 1,337,274 + -0.3 1,337,424 - +0.2
Or see this region's nucleotide sequence