Experiment: P. simiae ORA MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03795 and PS417_03800 are separated by 11 nucleotides PS417_03800 and PS417_03805 are separated by 62 nucleotides PS417_03805 and PS417_03810 overlap by 14 nucleotides PS417_03810 and PS417_03815 are separated by 124 nucleotides
PS417_03795: PS417_03795 - pilus assembly protein PilX, at 857,947 to 858,477
_03795
PS417_03800: PS417_03800 - pilus assembly protein, at 858,489 to 858,890
_03800
PS417_03805: PS417_03805 - D-amino acid oxidase, at 858,953 to 860,062
_03805
PS417_03810: PS417_03810 - hypothetical protein, at 860,049 to 860,282
_03810
PS417_03815: PS417_03815 - DNA transporter, at 860,407 to 861,432
_03815
Position (kb) 858
859
860
861 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 857.965 kb on - strand at 857.965 kb on - strand at 858.097 kb on - strand, within PS417_03795 at 858.259 kb on - strand, within PS417_03795 at 858.330 kb on - strand, within PS417_03795 at 858.412 kb on + strand at 858.413 kb on - strand at 858.423 kb on - strand, within PS417_03795 at 858.423 kb on - strand, within PS417_03795 at 858.423 kb on - strand, within PS417_03795 at 858.512 kb on + strand at 858.513 kb on - strand at 858.661 kb on - strand, within PS417_03800 at 858.681 kb on - strand at 858.681 kb on - strand, within PS417_03800 at 858.720 kb on - strand, within PS417_03800 at 858.720 kb on - strand, within PS417_03800 at 858.765 kb on - strand, within PS417_03800 at 858.972 kb on + strand at 859.113 kb on - strand, within PS417_03805 at 859.113 kb on - strand, within PS417_03805 at 859.124 kb on + strand, within PS417_03805 at 859.359 kb on - strand, within PS417_03805 at 859.373 kb on + strand, within PS417_03805 at 859.374 kb on - strand, within PS417_03805 at 859.506 kb on + strand, within PS417_03805 at 859.506 kb on + strand, within PS417_03805 at 859.507 kb on - strand, within PS417_03805 at 859.507 kb on - strand, within PS417_03805 at 859.592 kb on - strand, within PS417_03805 at 859.592 kb on - strand, within PS417_03805 at 859.592 kb on - strand, within PS417_03805 at 859.889 kb on + strand, within PS417_03805 at 859.889 kb on + strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 859.890 kb on - strand, within PS417_03805 at 860.061 kb on - strand at 860.099 kb on - strand, within PS417_03810 at 860.099 kb on - strand, within PS417_03810 at 860.239 kb on - strand at 860.348 kb on + strand at 860.349 kb on - strand at 860.349 kb on - strand at 860.349 kb on - strand at 860.433 kb on - strand at 860.433 kb on - strand at 860.552 kb on + strand, within PS417_03815 at 860.630 kb on + strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815 at 860.631 kb on - strand, within PS417_03815
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 10 remove 857,965 - +0.8 857,965 - +1.2 858,097 - PS417_03795 0.28 -0.1 858,259 - PS417_03795 0.59 -1.4 858,330 - PS417_03795 0.72 +0.1 858,412 + +2.4 858,413 - -1.4 858,423 - PS417_03795 0.90 -0.5 858,423 - PS417_03795 0.90 -0.2 858,423 - PS417_03795 0.90 -0.7 858,512 + +1.2 858,513 - -0.2 858,661 - PS417_03800 0.43 +0.2 858,681 - +1.4 858,681 - PS417_03800 0.48 -1.0 858,720 - PS417_03800 0.57 -0.8 858,720 - PS417_03800 0.57 -0.2 858,765 - PS417_03800 0.69 -0.4 858,972 + +0.0 859,113 - PS417_03805 0.14 +0.9 859,113 - PS417_03805 0.14 +0.4 859,124 + PS417_03805 0.15 +0.7 859,359 - PS417_03805 0.37 -0.6 859,373 + PS417_03805 0.38 -0.3 859,374 - PS417_03805 0.38 -0.2 859,506 + PS417_03805 0.50 +0.7 859,506 + PS417_03805 0.50 -2.0 859,507 - PS417_03805 0.50 +1.4 859,507 - PS417_03805 0.50 +2.3 859,592 - PS417_03805 0.58 +2.3 859,592 - PS417_03805 0.58 -0.2 859,592 - PS417_03805 0.58 +0.7 859,889 + PS417_03805 0.84 +0.4 859,889 + PS417_03805 0.84 -0.1 859,890 - PS417_03805 0.84 +2.3 859,890 - PS417_03805 0.84 +0.1 859,890 - PS417_03805 0.84 +2.1 859,890 - PS417_03805 0.84 +0.4 859,890 - PS417_03805 0.84 +0.2 860,061 - +0.3 860,099 - PS417_03810 0.21 +1.4 860,099 - PS417_03810 0.21 +0.0 860,239 - -0.6 860,348 + -0.7 860,349 - +0.9 860,349 - -0.1 860,349 - -1.6 860,433 - -3.7 860,433 - -1.4 860,552 + PS417_03815 0.14 -0.6 860,630 + PS417_03815 0.22 -2.0 860,631 - PS417_03815 0.22 +0.7 860,631 - PS417_03815 0.22 +0.1 860,631 - PS417_03815 0.22 -2.0 860,631 - PS417_03815 0.22 +1.7
Or see this region's nucleotide sequence