Experiment: P. simiae ORA MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07240 and PS417_07245 overlap by 4 nucleotides PS417_07245 and PS417_07250 are separated by 67 nucleotides PS417_07250 and PS417_07255 are separated by 65 nucleotides
PS417_07240: PS417_07240 - Transcriptional regulator of deoxyribose catabolism (from data) , at 1,597,407 to 1,598,129
_07240
PS417_07245: PS417_07245 - 2-deoxy-D-ribonate 3-dehydrogenase (from data) , at 1,598,126 to 1,598,914
_07245
PS417_07250: PS417_07250 - 2-deoxy-3-keto-D-ribonoate cleavage enzyme (from data) , at 1,598,982 to 1,599,914
_07250
PS417_07255: PS417_07255 - Deoxyribonolactonase (from data) , at 1,599,980 to 1,600,858
_07255
Position (kb)
1598
1599
1600 Strain fitness (log2 ratio)
-2
-1
0
1 at 1598.117 kb on + strand at 1598.136 kb on + strand at 1598.137 kb on - strand at 1598.164 kb on - strand at 1598.220 kb on - strand, within PS417_07245 at 1598.551 kb on + strand, within PS417_07245 at 1598.598 kb on - strand, within PS417_07245 at 1598.907 kb on - strand at 1598.907 kb on - strand at 1598.913 kb on - strand at 1598.963 kb on + strand at 1598.964 kb on - strand at 1598.964 kb on - strand at 1599.008 kb on + strand at 1599.009 kb on - strand at 1599.009 kb on - strand at 1599.036 kb on - strand at 1599.223 kb on + strand, within PS417_07250 at 1599.343 kb on - strand, within PS417_07250 at 1599.499 kb on - strand, within PS417_07250 at 1599.517 kb on - strand, within PS417_07250 at 1599.663 kb on + strand, within PS417_07250 at 1599.664 kb on - strand, within PS417_07250 at 1599.664 kb on - strand, within PS417_07250 at 1599.822 kb on - strand at 1600.455 kb on - strand, within PS417_07255 at 1600.490 kb on + strand, within PS417_07255 at 1600.490 kb on + strand, within PS417_07255 at 1600.496 kb on + strand, within PS417_07255 at 1600.602 kb on + strand, within PS417_07255 at 1600.619 kb on + strand, within PS417_07255 at 1600.879 kb on + strand at 1600.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae ORA MOI 10 remove 1,598,117 + +0.3 1,598,136 + +0.1 1,598,137 - +1.3 1,598,164 - +0.2 1,598,220 - PS417_07245 0.12 +0.8 1,598,551 + PS417_07245 0.54 +1.5 1,598,598 - PS417_07245 0.60 -0.4 1,598,907 - -0.7 1,598,907 - -0.1 1,598,913 - +0.5 1,598,963 + +0.1 1,598,964 - -0.1 1,598,964 - +0.6 1,599,008 + +0.2 1,599,009 - -0.7 1,599,009 - +0.8 1,599,036 - -1.7 1,599,223 + PS417_07250 0.26 -0.3 1,599,343 - PS417_07250 0.39 -2.0 1,599,499 - PS417_07250 0.55 -0.6 1,599,517 - PS417_07250 0.57 -1.8 1,599,663 + PS417_07250 0.73 -2.4 1,599,664 - PS417_07250 0.73 -2.6 1,599,664 - PS417_07250 0.73 +1.1 1,599,822 - -2.6 1,600,455 - PS417_07255 0.54 +1.1 1,600,490 + PS417_07255 0.58 -1.9 1,600,490 + PS417_07255 0.58 -0.4 1,600,496 + PS417_07255 0.59 -2.3 1,600,602 + PS417_07255 0.71 -0.2 1,600,619 + PS417_07255 0.73 +1.6 1,600,879 + +0.1 1,600,880 - -0.1
Or see this region's nucleotide sequence