Strain Fitness in Pseudomonas putida KT2440 around PP_5537

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2652 and PP_2653 overlap by 11 nucleotidesPP_2653 and PP_5537 are separated by 106 nucleotidesPP_5537 and PP_2654 overlap by 4 nucleotidesPP_2654 and PP_2655 are separated by 71 nucleotidesPP_2655 and PP_2656 are separated by 202 nucleotides PP_2652: PP_2652 - putative Hydratase/decarboxylase, at 3,040,398 to 3,041,183 _2652 PP_2653: PP_2653 - Transcriptional regulator, Cro/CI family, at 3,041,173 to 3,041,991 _2653 PP_5537: PP_5537 - conserved membrane protein of unknown function, at 3,042,098 to 3,042,412 _5537 PP_2654: PP_2654 - putative Glutathione S-transferase, at 3,042,409 to 3,042,834 _2654 PP_2655: PP_2655 - conserved protein of unknown function, at 3,042,906 to 3,043,139 _2655 PP_2656: PP_2656 - phosphate ABC transporter, at 3,043,342 to 3,044,388 _2656 Position (kb) 3042 3043Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3041.174 kb on + strandat 3041.181 kb on + strandat 3041.182 kb on - strandat 3041.182 kb on - strandat 3041.330 kb on + strand, within PP_2653at 3041.670 kb on + strand, within PP_2653at 3041.671 kb on - strand, within PP_2653at 3041.685 kb on + strand, within PP_2653at 3041.686 kb on - strand, within PP_2653at 3042.059 kb on - strandat 3042.064 kb on + strandat 3042.065 kb on - strandat 3042.095 kb on + strandat 3042.095 kb on + strandat 3042.095 kb on + strandat 3042.095 kb on + strandat 3042.095 kb on + strandat 3042.095 kb on + strandat 3042.096 kb on - strandat 3042.096 kb on - strandat 3042.097 kb on + strandat 3042.098 kb on - strandat 3042.126 kb on + strandat 3042.147 kb on - strand, within PP_5537at 3042.326 kb on + strand, within PP_5537at 3042.326 kb on + strand, within PP_5537at 3042.371 kb on + strand, within PP_5537at 3042.372 kb on - strand, within PP_5537at 3042.431 kb on - strandat 3042.581 kb on + strand, within PP_2654at 3042.582 kb on - strand, within PP_2654at 3042.582 kb on - strand, within PP_2654at 3042.582 kb on - strand, within PP_2654at 3042.596 kb on + strand, within PP_2654at 3042.597 kb on - strand, within PP_2654at 3042.683 kb on + strand, within PP_2654at 3042.683 kb on + strand, within PP_2654at 3042.684 kb on - strand, within PP_2654at 3042.684 kb on - strand, within PP_2654at 3042.684 kb on - strand, within PP_2654at 3042.734 kb on - strand, within PP_2654at 3042.734 kb on - strand, within PP_2654at 3042.769 kb on + strand, within PP_2654at 3042.872 kb on + strandat 3042.878 kb on + strandat 3042.878 kb on + strandat 3042.881 kb on + strandat 3042.888 kb on + strandat 3042.963 kb on + strand, within PP_2655at 3042.964 kb on - strand, within PP_2655at 3042.964 kb on - strand, within PP_2655at 3043.005 kb on + strandat 3043.014 kb on + strand, within PP_2655at 3043.116 kb on - strandat 3043.188 kb on + strandat 3043.266 kb on - strandat 3043.266 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
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3,041,174 + +0.3
3,041,181 + -1.4
3,041,182 - -0.7
3,041,182 - -0.8
3,041,330 + PP_2653 0.19 -0.5
3,041,670 + PP_2653 0.61 -2.4
3,041,671 - PP_2653 0.61 +1.2
3,041,685 + PP_2653 0.63 +0.5
3,041,686 - PP_2653 0.63 +1.0
3,042,059 - +0.3
3,042,064 + -0.1
3,042,065 - +0.1
3,042,095 + -1.6
3,042,095 + -0.9
3,042,095 + -0.7
3,042,095 + -2.4
3,042,095 + -0.1
3,042,095 + -2.2
3,042,096 - -2.8
3,042,096 - +1.6
3,042,097 + +1.6
3,042,098 - -1.4
3,042,126 + +0.0
3,042,147 - PP_5537 0.16 +2.4
3,042,326 + PP_5537 0.72 +1.0
3,042,326 + PP_5537 0.72 +0.6
3,042,371 + PP_5537 0.87 -4.4
3,042,372 - PP_5537 0.87 +1.1
3,042,431 - -2.9
3,042,581 + PP_2654 0.40 +0.5
3,042,582 - PP_2654 0.41 +0.7
3,042,582 - PP_2654 0.41 +0.8
3,042,582 - PP_2654 0.41 +0.5
3,042,596 + PP_2654 0.44 -3.7
3,042,597 - PP_2654 0.44 +1.7
3,042,683 + PP_2654 0.64 +0.6
3,042,683 + PP_2654 0.64 +0.5
3,042,684 - PP_2654 0.65 +0.6
3,042,684 - PP_2654 0.65 +0.2
3,042,684 - PP_2654 0.65 +0.9
3,042,734 - PP_2654 0.76 +1.0
3,042,734 - PP_2654 0.76 +0.6
3,042,769 + PP_2654 0.85 -0.5
3,042,872 + +0.7
3,042,878 + +1.6
3,042,878 + -1.1
3,042,881 + +1.6
3,042,888 + +0.8
3,042,963 + PP_2655 0.24 +0.3
3,042,964 - PP_2655 0.25 -1.4
3,042,964 - PP_2655 0.25 -4.0
3,043,005 + -1.6
3,043,014 + PP_2655 0.46 +0.2
3,043,116 - +1.5
3,043,188 + -0.0
3,043,266 - +0.5
3,043,266 - +0.9

Or see this region's nucleotide sequence