Strain Fitness in Pseudomonas putida KT2440 around PP_4609

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4607 and PP_4608 overlap by 4 nucleotidesPP_4608 and PP_4609 are separated by 5 nucleotidesPP_4609 and PP_4610 overlap by 1 nucleotides PP_4607: PP_4607 - putative Transmembrane sensor, at 5,229,234 to 5,230,214 _4607 PP_4608: PP_4608 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,230,211 to 5,230,786 _4608 PP_4609: PP_4609 - conserved membrane protein of unknown function, at 5,230,792 to 5,231,118 _4609 PP_4610: PP_4610 - conserved protein of unknown function containing PepSY-associated TM helix domain, at 5,231,118 to 5,232,695 _4610 Position (kb) 5230 5231 5232Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5229.844 kb on + strand, within PP_4607at 5229.845 kb on - strand, within PP_4607at 5229.845 kb on - strand, within PP_4607at 5230.072 kb on + strand, within PP_4607at 5230.294 kb on + strand, within PP_4608at 5230.295 kb on - strand, within PP_4608at 5230.295 kb on - strand, within PP_4608at 5230.295 kb on - strand, within PP_4608at 5230.295 kb on - strand, within PP_4608at 5230.295 kb on - strand, within PP_4608at 5230.347 kb on + strand, within PP_4608at 5230.348 kb on - strandat 5230.423 kb on + strand, within PP_4608at 5230.522 kb on + strand, within PP_4608at 5230.544 kb on + strand, within PP_4608at 5230.544 kb on + strand, within PP_4608at 5230.544 kb on + strand, within PP_4608at 5230.545 kb on - strand, within PP_4608at 5230.545 kb on - strand, within PP_4608at 5230.575 kb on + strand, within PP_4608at 5230.575 kb on + strand, within PP_4608at 5230.575 kb on + strand, within PP_4608at 5230.576 kb on - strandat 5230.679 kb on + strand, within PP_4608at 5230.680 kb on - strand, within PP_4608at 5230.976 kb on - strand, within PP_4609at 5231.083 kb on + strand, within PP_4609at 5231.083 kb on + strand, within PP_4609at 5231.083 kb on + strandat 5231.083 kb on + strand, within PP_4609at 5231.083 kb on + strand, within PP_4609at 5231.084 kb on - strand, within PP_4609at 5231.084 kb on - strand, within PP_4609at 5231.084 kb on - strand, within PP_4609at 5231.084 kb on - strand, within PP_4609at 5231.179 kb on - strandat 5231.283 kb on + strand, within PP_4610at 5231.391 kb on - strand, within PP_4610at 5231.540 kb on + strand, within PP_4610at 5231.540 kb on + strand, within PP_4610at 5231.576 kb on + strand, within PP_4610at 5231.576 kb on + strand, within PP_4610at 5231.576 kb on + strand, within PP_4610at 5231.576 kb on + strand, within PP_4610at 5231.577 kb on - strand, within PP_4610at 5231.577 kb on - strand, within PP_4610at 5231.577 kb on - strand, within PP_4610at 5231.671 kb on + strand, within PP_4610at 5231.671 kb on + strand, within PP_4610at 5231.671 kb on + strand, within PP_4610at 5231.672 kb on - strand, within PP_4610at 5231.672 kb on - strand, within PP_4610at 5231.672 kb on - strand, within PP_4610at 5231.743 kb on - strand, within PP_4610at 5231.936 kb on - strand, within PP_4610at 5232.017 kb on - strand, within PP_4610at 5232.017 kb on - strand, within PP_4610at 5232.017 kb on - strand, within PP_4610at 5232.037 kb on + strand, within PP_4610at 5232.038 kb on - strand, within PP_4610at 5232.070 kb on + strand, within PP_4610at 5232.070 kb on + strand, within PP_4610at 5232.071 kb on - strand, within PP_4610at 5232.071 kb on - strand, within PP_4610at 5232.071 kb on - strand, within PP_4610at 5232.071 kb on - strand, within PP_4610at 5232.094 kb on + strand, within PP_4610at 5232.094 kb on + strand, within PP_4610at 5232.095 kb on - strand, within PP_4610at 5232.095 kb on - strand, within PP_4610

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
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5,229,844 + PP_4607 0.62 +1.4
5,229,845 - PP_4607 0.62 +0.9
5,229,845 - PP_4607 0.62 -3.9
5,230,072 + PP_4607 0.85 +0.1
5,230,294 + PP_4608 0.14 -0.3
5,230,295 - PP_4608 0.15 -2.0
5,230,295 - PP_4608 0.15 +0.2
5,230,295 - PP_4608 0.15 -3.5
5,230,295 - PP_4608 0.15 +0.8
5,230,295 - PP_4608 0.15 +0.4
5,230,347 + PP_4608 0.24 -1.0
5,230,348 - +0.5
5,230,423 + PP_4608 0.37 +0.7
5,230,522 + PP_4608 0.54 -1.9
5,230,544 + PP_4608 0.58 +1.5
5,230,544 + PP_4608 0.58 +0.4
5,230,544 + PP_4608 0.58 +0.1
5,230,545 - PP_4608 0.58 -3.2
5,230,545 - PP_4608 0.58 +2.6
5,230,575 + PP_4608 0.63 +2.3
5,230,575 + PP_4608 0.63 -0.5
5,230,575 + PP_4608 0.63 -1.1
5,230,576 - -1.1
5,230,679 + PP_4608 0.81 -1.2
5,230,680 - PP_4608 0.81 -2.9
5,230,976 - PP_4609 0.56 +1.0
5,231,083 + PP_4609 0.89 -0.3
5,231,083 + PP_4609 0.89 +0.4
5,231,083 + -0.2
5,231,083 + PP_4609 0.89 +1.2
5,231,083 + PP_4609 0.89 +1.3
5,231,084 - PP_4609 0.89 +1.7
5,231,084 - PP_4609 0.89 +0.1
5,231,084 - PP_4609 0.89 +0.6
5,231,084 - PP_4609 0.89 +1.2
5,231,179 - -3.7
5,231,283 + PP_4610 0.10 +0.4
5,231,391 - PP_4610 0.17 +0.8
5,231,540 + PP_4610 0.27 +0.9
5,231,540 + PP_4610 0.27 +2.1
5,231,576 + PP_4610 0.29 -0.8
5,231,576 + PP_4610 0.29 +0.6
5,231,576 + PP_4610 0.29 -1.3
5,231,576 + PP_4610 0.29 +1.2
5,231,577 - PP_4610 0.29 -0.3
5,231,577 - PP_4610 0.29 +1.1
5,231,577 - PP_4610 0.29 +1.2
5,231,671 + PP_4610 0.35 +0.1
5,231,671 + PP_4610 0.35 +0.6
5,231,671 + PP_4610 0.35 +0.4
5,231,672 - PP_4610 0.35 -3.0
5,231,672 - PP_4610 0.35 +0.7
5,231,672 - PP_4610 0.35 +0.6
5,231,743 - PP_4610 0.40 -0.0
5,231,936 - PP_4610 0.52 +1.0
5,232,017 - PP_4610 0.57 +0.9
5,232,017 - PP_4610 0.57 -2.4
5,232,017 - PP_4610 0.57 +0.5
5,232,037 + PP_4610 0.58 -1.1
5,232,038 - PP_4610 0.58 -0.1
5,232,070 + PP_4610 0.60 -0.1
5,232,070 + PP_4610 0.60 -0.0
5,232,071 - PP_4610 0.60 +0.3
5,232,071 - PP_4610 0.60 -1.3
5,232,071 - PP_4610 0.60 -4.6
5,232,071 - PP_4610 0.60 -3.7
5,232,094 + PP_4610 0.62 -1.1
5,232,094 + PP_4610 0.62 -1.1
5,232,095 - PP_4610 0.62 -0.6
5,232,095 - PP_4610 0.62 +0.2

Or see this region's nucleotide sequence