Strain Fitness in Pseudomonas putida KT2440 around PP_2749

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_2747 and PP_2748 overlap by 34 nucleotidesPP_2748 and PP_2749 overlap by 4 nucleotidesPP_2749 and PP_2750 are separated by 4 nucleotides PP_2747: PP_2747 - conserved protein of unknown function, at 3,130,184 to 3,131,155 _2747 PP_2748: PP_2748 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,131,122 to 3,131,922 _2748 PP_2749: PP_2749 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,131,919 to 3,132,800 _2749 PP_2750: PP_2750 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,132,805 to 3,133,869 _2750 Position (kb) 3131 3132 3133Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3131.080 kb on + strandat 3131.081 kb on - strandat 3131.081 kb on - strandat 3131.081 kb on - strandat 3131.081 kb on - strandat 3131.101 kb on - strandat 3131.135 kb on + strandat 3131.312 kb on - strand, within PP_2748at 3131.615 kb on - strand, within PP_2748at 3131.841 kb on + strand, within PP_2748at 3131.841 kb on + strand, within PP_2748at 3132.003 kb on + strandat 3132.003 kb on + strandat 3132.122 kb on - strand, within PP_2749at 3132.269 kb on - strand, within PP_2749at 3132.574 kb on + strand, within PP_2749at 3132.574 kb on + strand, within PP_2749at 3132.682 kb on - strand, within PP_2749at 3132.751 kb on - strandat 3132.751 kb on - strandat 3132.751 kb on - strandat 3132.751 kb on - strandat 3132.797 kb on - strandat 3133.265 kb on - strand, within PP_2750at 3133.484 kb on - strand, within PP_2750at 3133.484 kb on - strand, within PP_2750at 3133.517 kb on - strand, within PP_2750

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
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3,131,080 + +0.6
3,131,081 - -0.8
3,131,081 - +1.4
3,131,081 - +1.0
3,131,081 - -0.5
3,131,101 - -0.7
3,131,135 + +1.1
3,131,312 - PP_2748 0.24 +1.3
3,131,615 - PP_2748 0.62 -2.8
3,131,841 + PP_2748 0.90 -0.3
3,131,841 + PP_2748 0.90 +0.8
3,132,003 + +0.5
3,132,003 + -0.5
3,132,122 - PP_2749 0.23 +1.5
3,132,269 - PP_2749 0.40 -0.9
3,132,574 + PP_2749 0.74 +1.2
3,132,574 + PP_2749 0.74 -0.1
3,132,682 - PP_2749 0.87 +2.1
3,132,751 - +0.2
3,132,751 - +0.3
3,132,751 - -0.2
3,132,751 - +0.8
3,132,797 - +1.4
3,133,265 - PP_2750 0.43 -0.7
3,133,484 - PP_2750 0.64 -0.6
3,133,484 - PP_2750 0.64 -0.5
3,133,517 - PP_2750 0.67 +0.1

Or see this region's nucleotide sequence