Strain Fitness in Pseudomonas putida KT2440 around PP_1647

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1644 and PP_1645 overlap by 4 nucleotidesPP_1645 and PP_1646 are separated by 85 nucleotidesPP_1646 and PP_1647 are separated by 35 nucleotidesPP_1647 and PP_1648 overlap by 31 nucleotidesPP_1648 and PP_1649 are separated by 31 nucleotides PP_1644: PP_1644 - NAD(P)H dehydrogenase (quinone), at 1,838,563 to 1,839,168 _1644 PP_1645: PP_1645 - putative enzyme, at 1,839,165 to 1,839,518 _1645 PP_1646: PP_1646 - putative Thioredoxin, at 1,839,604 to 1,840,068 _1646 PP_1647: PP_1647 - conserved protein of unknown function, at 1,840,104 to 1,840,535 _1647 PP_1648: PP_1648 - conserved protein of unknown function, at 1,840,505 to 1,840,942 _1648 PP_1649: PP_1649 - D-lactate dehydrogenase, at 1,840,974 to 1,842,002 _1649 Position (kb) 1840 1841Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1839.137 kb on + strandat 1839.138 kb on - strandat 1839.138 kb on - strandat 1839.140 kb on + strandat 1839.141 kb on - strandat 1839.156 kb on - strandat 1839.320 kb on + strand, within PP_1645at 1839.320 kb on + strand, within PP_1645at 1839.320 kb on + strand, within PP_1645at 1839.320 kb on + strand, within PP_1645at 1839.321 kb on - strand, within PP_1645at 1839.321 kb on - strand, within PP_1645at 1839.321 kb on - strand, within PP_1645at 1839.322 kb on + strand, within PP_1645at 1839.323 kb on - strand, within PP_1645at 1839.418 kb on + strand, within PP_1645at 1839.419 kb on - strand, within PP_1645at 1839.494 kb on + strandat 1839.495 kb on - strandat 1839.500 kb on - strandat 1839.500 kb on - strandat 1839.554 kb on + strandat 1839.555 kb on - strandat 1839.555 kb on - strandat 1839.627 kb on - strandat 1839.693 kb on + strand, within PP_1646at 1839.696 kb on + strand, within PP_1646at 1839.697 kb on - strand, within PP_1646at 1839.697 kb on - strand, within PP_1646at 1839.697 kb on - strand, within PP_1646at 1839.970 kb on + strand, within PP_1646at 1840.192 kb on - strand, within PP_1647at 1840.340 kb on - strand, within PP_1647at 1840.381 kb on + strand, within PP_1647at 1840.382 kb on - strand, within PP_1647at 1840.420 kb on + strand, within PP_1647at 1840.421 kb on - strand, within PP_1647at 1840.421 kb on - strand, within PP_1647at 1840.921 kb on - strandat 1840.969 kb on + strandat 1840.970 kb on - strandat 1840.976 kb on - strandat 1841.186 kb on - strand, within PP_1649at 1841.382 kb on + strand, within PP_1649at 1841.447 kb on - strand, within PP_1649

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
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1,839,137 + +0.9
1,839,138 - +1.4
1,839,138 - +0.6
1,839,140 + +1.4
1,839,141 - -0.3
1,839,156 - +0.9
1,839,320 + PP_1645 0.44 +0.8
1,839,320 + PP_1645 0.44 +1.8
1,839,320 + PP_1645 0.44 -0.8
1,839,320 + PP_1645 0.44 +0.2
1,839,321 - PP_1645 0.44 +0.5
1,839,321 - PP_1645 0.44 -3.1
1,839,321 - PP_1645 0.44 +1.8
1,839,322 + PP_1645 0.44 -0.2
1,839,323 - PP_1645 0.45 +0.6
1,839,418 + PP_1645 0.71 +0.1
1,839,419 - PP_1645 0.72 +0.2
1,839,494 + +1.0
1,839,495 - -1.2
1,839,500 - +0.7
1,839,500 - -0.6
1,839,554 + -2.8
1,839,555 - +1.2
1,839,555 - +0.8
1,839,627 - +0.5
1,839,693 + PP_1646 0.19 +0.8
1,839,696 + PP_1646 0.20 +0.5
1,839,697 - PP_1646 0.20 -0.1
1,839,697 - PP_1646 0.20 -0.5
1,839,697 - PP_1646 0.20 -0.1
1,839,970 + PP_1646 0.79 -0.2
1,840,192 - PP_1647 0.20 +1.9
1,840,340 - PP_1647 0.55 +1.2
1,840,381 + PP_1647 0.64 +1.2
1,840,382 - PP_1647 0.64 +1.4
1,840,420 + PP_1647 0.73 +0.3
1,840,421 - PP_1647 0.73 +1.5
1,840,421 - PP_1647 0.73 +1.1
1,840,921 - +2.0
1,840,969 + -0.2
1,840,970 - +0.2
1,840,976 - +0.1
1,841,186 - PP_1649 0.21 +0.2
1,841,382 + PP_1649 0.40 -0.8
1,841,447 - PP_1649 0.46 -0.8

Or see this region's nucleotide sequence