Strain Fitness in Pseudomonas putida KT2440 around PP_4627

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4625 and PP_4626 overlap by 11 nucleotidesPP_4626 and PP_4627 are separated by 97 nucleotidesPP_4627 and PP_4628 are separated by 21 nucleotidesPP_4628 and PP_4629 are separated by 115 nucleotides PP_4625: PP_4625 - anti-holin regulator of holin CidA, at 5,249,998 to 5,250,684 _4625 PP_4626: PP_4626 - holin regulator of murein hydrolases, at 5,250,674 to 5,251,060 _4626 PP_4627: PP_4627 - transcriptional regulator, LysR family, at 5,251,158 to 5,252,039 _4627 PP_4628: PP_4628 - conserved protein of unknown function, at 5,252,061 to 5,252,516 _4628 PP_4629: PP_4629 - Sensory box protein, at 5,252,632 to 5,253,060 _4629 Position (kb) 5251 5252 5253Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5250.162 kb on + strand, within PP_4625at 5250.524 kb on + strand, within PP_4625at 5250.594 kb on - strand, within PP_4625at 5250.611 kb on + strand, within PP_4625at 5250.612 kb on - strand, within PP_4625at 5250.612 kb on - strand, within PP_4625at 5250.632 kb on + strandat 5250.731 kb on + strand, within PP_4626at 5250.731 kb on + strand, within PP_4626at 5250.731 kb on + strand, within PP_4626at 5250.732 kb on - strand, within PP_4626at 5250.741 kb on - strand, within PP_4626at 5250.741 kb on - strand, within PP_4626at 5250.804 kb on + strand, within PP_4626at 5250.914 kb on + strand, within PP_4626at 5250.914 kb on + strand, within PP_4626at 5250.914 kb on + strand, within PP_4626at 5250.915 kb on - strand, within PP_4626at 5250.915 kb on - strand, within PP_4626at 5251.046 kb on + strandat 5251.046 kb on + strandat 5251.046 kb on + strandat 5251.047 kb on - strandat 5251.089 kb on + strandat 5251.435 kb on + strand, within PP_4627at 5251.436 kb on - strand, within PP_4627at 5251.436 kb on - strand, within PP_4627at 5251.436 kb on - strand, within PP_4627at 5251.624 kb on - strand, within PP_4627at 5251.719 kb on - strand, within PP_4627at 5251.734 kb on + strand, within PP_4627at 5251.735 kb on - strand, within PP_4627at 5251.965 kb on - strandat 5252.215 kb on + strand, within PP_4628at 5252.216 kb on - strand, within PP_4628at 5252.216 kb on - strand, within PP_4628at 5252.219 kb on + strand, within PP_4628at 5252.219 kb on + strand, within PP_4628at 5252.227 kb on + strand, within PP_4628at 5252.348 kb on + strand, within PP_4628at 5252.486 kb on - strandat 5252.502 kb on - strandat 5252.725 kb on + strand, within PP_4629at 5252.725 kb on + strand, within PP_4629at 5252.746 kb on + strand, within PP_4629at 5252.758 kb on + strand, within PP_4629at 5252.759 kb on - strand, within PP_4629at 5252.887 kb on + strand, within PP_4629at 5252.888 kb on - strand, within PP_4629at 5252.962 kb on + strand, within PP_4629at 5252.962 kb on + strand, within PP_4629at 5252.963 kb on - strand, within PP_4629

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
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5,250,162 + PP_4625 0.24 -0.2
5,250,524 + PP_4625 0.77 -0.1
5,250,594 - PP_4625 0.87 +0.8
5,250,611 + PP_4625 0.89 +1.4
5,250,612 - PP_4625 0.89 +1.0
5,250,612 - PP_4625 0.89 -3.8
5,250,632 + +0.9
5,250,731 + PP_4626 0.15 -0.0
5,250,731 + PP_4626 0.15 -0.3
5,250,731 + PP_4626 0.15 +0.0
5,250,732 - PP_4626 0.15 +0.3
5,250,741 - PP_4626 0.17 +0.8
5,250,741 - PP_4626 0.17 -0.4
5,250,804 + PP_4626 0.34 +0.6
5,250,914 + PP_4626 0.62 +0.4
5,250,914 + PP_4626 0.62 -4.4
5,250,914 + PP_4626 0.62 +1.5
5,250,915 - PP_4626 0.62 +1.2
5,250,915 - PP_4626 0.62 -3.1
5,251,046 + +1.1
5,251,046 + +0.2
5,251,046 + -4.4
5,251,047 - -4.5
5,251,089 + -2.0
5,251,435 + PP_4627 0.31 -2.6
5,251,436 - PP_4627 0.32 +1.0
5,251,436 - PP_4627 0.32 +0.4
5,251,436 - PP_4627 0.32 +1.1
5,251,624 - PP_4627 0.53 -3.7
5,251,719 - PP_4627 0.64 +2.8
5,251,734 + PP_4627 0.65 +0.5
5,251,735 - PP_4627 0.65 -0.2
5,251,965 - +0.9
5,252,215 + PP_4628 0.34 -1.8
5,252,216 - PP_4628 0.34 +0.5
5,252,216 - PP_4628 0.34 +0.2
5,252,219 + PP_4628 0.35 +0.8
5,252,219 + PP_4628 0.35 +1.4
5,252,227 + PP_4628 0.36 +1.1
5,252,348 + PP_4628 0.63 -1.1
5,252,486 - -1.5
5,252,502 - -1.3
5,252,725 + PP_4629 0.22 +1.5
5,252,725 + PP_4629 0.22 -0.5
5,252,746 + PP_4629 0.27 +1.9
5,252,758 + PP_4629 0.29 -3.3
5,252,759 - PP_4629 0.30 -4.3
5,252,887 + PP_4629 0.59 -1.7
5,252,888 - PP_4629 0.60 +1.0
5,252,962 + PP_4629 0.77 +0.4
5,252,962 + PP_4629 0.77 -0.8
5,252,963 - PP_4629 0.77 -0.1

Or see this region's nucleotide sequence