Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1898 and PP_1899 overlap by 4 nucleotides PP_1899 and PP_1900 overlap by 4 nucleotides PP_1900 and PP_1901 are separated by 44 nucleotides PP_1901 and PP_1902 overlap by 4 nucleotides PP_1902 and PP_1903 overlap by 1 nucleotides
PP_1898: PP_1898 - MotA/TolQ/ExbB proton channel family protein, at 2,142,550 to 2,143,224
_1898
PP_1899: PP_1899 - ExbD/TolR family protein, at 2,143,221 to 2,143,655
_1899
PP_1900: PP_1900 - Tetraacyldisaccharide 4'-kinase, at 2,143,652 to 2,144,656
_1900
PP_1901: PP_1901 - conserved protein of unknown function, UPF0434 family, at 2,144,701 to 2,144,886
_1901
PP_1902: PP_1902 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,144,883 to 2,145,647
_1902
PP_1903: PP_1903 - putative phosphotyrosine-protein phosphatase, at 2,145,647 to 2,146,111
_1903
Position (kb)
2143
2144
2145 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2142.660 kb on + strand, within PP_1898 at 2142.728 kb on + strand, within PP_1898 at 2142.728 kb on + strand, within PP_1898 at 2142.904 kb on + strand, within PP_1898 at 2143.041 kb on + strand, within PP_1898 at 2143.086 kb on + strand, within PP_1898 at 2143.316 kb on + strand, within PP_1899 at 2144.686 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs remove 2,142,660 + PP_1898 0.16 -3.1 2,142,728 + PP_1898 0.26 -2.9 2,142,728 + PP_1898 0.26 -2.8 2,142,904 + PP_1898 0.52 -2.9 2,143,041 + PP_1898 0.73 -3.2 2,143,086 + PP_1898 0.79 -2.7 2,143,316 + PP_1899 0.22 -3.3 2,144,686 + -0.3
Or see this region's nucleotide sequence