Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0702 and PP_0703 are separated by 7 nucleotides PP_0703 and PP_0704 overlap by 8 nucleotides PP_0704 and PP_0705 are separated by 171 nucleotides
PP_0702: PP_0702 - Major facilitator family transporter, at 816,052 to 817,209
_0702
PP_0703: PP_0703 - putative Transmembrane sensor, at 817,217 to 818,167
_0703
PP_0704: PP_0704 - RNA polymerase sigma-70 factor, ECF subfamily, at 818,160 to 818,675
_0704
PP_0705: PP_0705 - DNA-3-methyladenine glycosylase, at 818,847 to 819,473
_0705
Position (kb)
817
818
819 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 816.265 kb on + strand, within PP_0702 at 816.265 kb on + strand, within PP_0702 at 816.265 kb on + strand, within PP_0702 at 816.265 kb on + strand, within PP_0702 at 816.265 kb on + strand, within PP_0702 at 816.266 kb on - strand, within PP_0702 at 816.266 kb on - strand, within PP_0702 at 816.266 kb on - strand, within PP_0702 at 816.266 kb on - strand, within PP_0702 at 816.266 kb on - strand, within PP_0702 at 816.266 kb on - strand, within PP_0702 at 816.266 kb on - strand, within PP_0702 at 816.451 kb on + strand, within PP_0702 at 816.452 kb on - strand, within PP_0702 at 816.452 kb on - strand, within PP_0702 at 816.452 kb on - strand, within PP_0702 at 816.724 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.967 kb on + strand, within PP_0702 at 816.968 kb on - strand, within PP_0702 at 816.968 kb on - strand, within PP_0702 at 816.968 kb on - strand, within PP_0702 at 816.968 kb on - strand, within PP_0702 at 816.968 kb on - strand, within PP_0702 at 816.968 kb on - strand, within PP_0702 at 816.968 kb on - strand at 816.968 kb on - strand, within PP_0702 at 816.968 kb on - strand, within PP_0702 at 816.969 kb on + strand, within PP_0702 at 816.969 kb on + strand, within PP_0702 at 816.969 kb on + strand, within PP_0702 at 816.969 kb on + strand, within PP_0702 at 816.969 kb on + strand, within PP_0702 at 816.970 kb on - strand, within PP_0702 at 816.970 kb on - strand, within PP_0702 at 816.970 kb on - strand, within PP_0702 at 816.970 kb on - strand, within PP_0702 at 817.210 kb on + strand at 817.218 kb on + strand at 817.219 kb on - strand at 817.219 kb on - strand at 817.242 kb on + strand at 817.242 kb on + strand at 817.243 kb on - strand at 817.243 kb on - strand at 817.255 kb on - strand at 817.320 kb on + strand, within PP_0703 at 817.434 kb on - strand, within PP_0703 at 817.740 kb on + strand, within PP_0703 at 817.740 kb on + strand, within PP_0703 at 817.740 kb on + strand, within PP_0703 at 818.011 kb on + strand, within PP_0703 at 818.156 kb on - strand at 818.173 kb on + strand at 818.174 kb on - strand at 818.351 kb on + strand, within PP_0704 at 818.369 kb on + strand, within PP_0704 at 818.369 kb on + strand, within PP_0704 at 818.370 kb on - strand, within PP_0704 at 818.370 kb on - strand, within PP_0704 at 818.370 kb on - strand, within PP_0704 at 818.488 kb on + strand, within PP_0704 at 818.488 kb on + strand, within PP_0704 at 818.489 kb on - strand, within PP_0704 at 818.546 kb on + strand, within PP_0704 at 818.615 kb on + strand, within PP_0704 at 818.616 kb on - strand, within PP_0704 at 818.616 kb on - strand, within PP_0704 at 818.630 kb on - strand at 818.678 kb on + strand at 818.679 kb on - strand at 818.742 kb on + strand at 818.743 kb on - strand at 818.743 kb on - strand at 818.770 kb on + strand at 818.770 kb on + strand at 818.771 kb on - strand at 818.812 kb on + strand at 818.878 kb on - strand at 818.930 kb on + strand, within PP_0705 at 818.964 kb on - strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.982 kb on + strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 818.983 kb on - strand, within PP_0705 at 818.983 kb on - strand, within PP_0705
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs remove 816,265 + PP_0702 0.18 +1.8 816,265 + PP_0702 0.18 +1.2 816,265 + PP_0702 0.18 +0.4 816,265 + PP_0702 0.18 -0.2 816,265 + PP_0702 0.18 +0.1 816,266 - PP_0702 0.18 -2.9 816,266 - PP_0702 0.18 +1.3 816,266 - PP_0702 0.18 -2.0 816,266 - PP_0702 0.18 +0.4 816,266 - PP_0702 0.18 +1.5 816,266 - PP_0702 0.18 +2.2 816,266 - PP_0702 0.18 +0.9 816,451 + PP_0702 0.34 +1.2 816,452 - PP_0702 0.35 -2.8 816,452 - PP_0702 0.35 +1.0 816,452 - PP_0702 0.35 -4.0 816,724 + PP_0702 0.58 -1.4 816,967 + PP_0702 0.79 -4.0 816,967 + PP_0702 0.79 +0.5 816,967 + PP_0702 0.79 +1.0 816,967 + PP_0702 0.79 +1.1 816,967 + PP_0702 0.79 +0.5 816,967 + PP_0702 0.79 -1.0 816,967 + PP_0702 0.79 +2.2 816,967 + PP_0702 0.79 +0.2 816,967 + PP_0702 0.79 -1.7 816,967 + PP_0702 0.79 -3.0 816,968 - PP_0702 0.79 -3.9 816,968 - PP_0702 0.79 +1.0 816,968 - PP_0702 0.79 -0.2 816,968 - PP_0702 0.79 -0.2 816,968 - PP_0702 0.79 -1.1 816,968 - PP_0702 0.79 -1.3 816,968 - -1.2 816,968 - PP_0702 0.79 -2.4 816,968 - PP_0702 0.79 +0.3 816,969 + PP_0702 0.79 +0.4 816,969 + PP_0702 0.79 -0.2 816,969 + PP_0702 0.79 -1.1 816,969 + PP_0702 0.79 -0.9 816,969 + PP_0702 0.79 +0.5 816,970 - PP_0702 0.79 -0.4 816,970 - PP_0702 0.79 +0.8 816,970 - PP_0702 0.79 +1.5 816,970 - PP_0702 0.79 -1.2 817,210 + -1.8 817,218 + +0.5 817,219 - +0.1 817,219 - -1.1 817,242 + +2.1 817,242 + -3.0 817,243 - -0.8 817,243 - +0.1 817,255 - +0.8 817,320 + PP_0703 0.11 -4.0 817,434 - PP_0703 0.23 +1.8 817,740 + PP_0703 0.55 -1.9 817,740 + PP_0703 0.55 +1.0 817,740 + PP_0703 0.55 +1.4 818,011 + PP_0703 0.83 +0.8 818,156 - -1.7 818,173 + -0.5 818,174 - -3.8 818,351 + PP_0704 0.37 +1.4 818,369 + PP_0704 0.41 -2.1 818,369 + PP_0704 0.41 -1.5 818,370 - PP_0704 0.41 +1.0 818,370 - PP_0704 0.41 +1.5 818,370 - PP_0704 0.41 -3.3 818,488 + PP_0704 0.64 -0.2 818,488 + PP_0704 0.64 +1.8 818,489 - PP_0704 0.64 -0.0 818,546 + PP_0704 0.75 -0.1 818,615 + PP_0704 0.88 +0.9 818,616 - PP_0704 0.88 -0.6 818,616 - PP_0704 0.88 -0.5 818,630 - +0.3 818,678 + -0.3 818,679 - +1.8 818,742 + +1.1 818,743 - +1.1 818,743 - +0.5 818,770 + +0.2 818,770 + -0.6 818,771 - -3.6 818,812 + +1.8 818,878 - -2.6 818,930 + PP_0705 0.13 -1.7 818,964 - PP_0705 0.19 +0.1 818,982 + PP_0705 0.22 -1.6 818,982 + PP_0705 0.22 -1.1 818,982 + PP_0705 0.22 -0.2 818,983 - PP_0705 0.22 -4.0 818,983 - PP_0705 0.22 +0.4 818,983 - PP_0705 0.22 -0.1
Or see this region's nucleotide sequence