Experiment: P. simiae OR Antrim MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01305 and PS417_01310 overlap by 4 nucleotides PS417_01310 and PS417_01315 are separated by 36 nucleotides PS417_01315 and PS417_01320 overlap by 4 nucleotides
PS417_01305: PS417_01305 - Fis family transcriptional regulator, at 282,243 to 283,619
_01305
PS417_01310: PS417_01310 - ATPase, at 283,616 to 285,424
_01310
PS417_01315: PS417_01315 - dTDP-4-dehydrorhamnose reductase, at 285,461 to 286,330
_01315
PS417_01320: PS417_01320 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 286,327 to 286,872
_01320
Position (kb) 283
284
285
286 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 282.701 kb on - strand, within PS417_01305 at 282.703 kb on + strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 283.315 kb on + strand, within PS417_01305 at 283.619 kb on + strand at 283.619 kb on + strand at 283.619 kb on + strand at 283.620 kb on - strand at 283.620 kb on - strand at 283.620 kb on - strand at 283.800 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 284.941 kb on - strand, within PS417_01310 at 285.102 kb on + strand, within PS417_01310 at 285.456 kb on + strand at 285.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. simiae OR Antrim MOI 0.1 remove 282,701 - PS417_01305 0.33 +0.4 282,703 + PS417_01305 0.33 -0.5 282,704 - PS417_01305 0.33 +0.3 282,704 - PS417_01305 0.33 -0.4 282,704 - PS417_01305 0.33 -1.7 283,315 + PS417_01305 0.78 -0.8 283,619 + +0.2 283,619 + +0.3 283,619 + +1.5 283,620 - -0.5 283,620 - -1.7 283,620 - -0.6 283,800 + PS417_01310 0.10 -0.0 283,863 + PS417_01310 0.14 +0.1 283,863 + PS417_01310 0.14 -2.1 283,863 + PS417_01310 0.14 -0.5 284,941 - PS417_01310 0.73 +1.0 285,102 + PS417_01310 0.82 -2.9 285,456 + -0.3 285,457 - -1.3
Or see this region's nucleotide sequence