Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3474 and PP_3475 overlap by 4 nucleotides PP_3475 and PP_3476 overlap by 29 nucleotides PP_3476 and PP_3477 overlap by 7 nucleotides PP_3477 and PP_3478 overlap by 4 nucleotides
PP_3474: PP_3474 - putative Curli fiber protein CsgE, at 3,940,271 to 3,940,672
_3474
PP_3475: PP_3475 - putative type II secretory pathway, pseudopilin pulG-related signal peptide protein, at 3,940,669 to 3,941,199
_3475
PP_3476: PP_3476 - Type II secretion system protein G, at 3,941,171 to 3,941,557
_3476
PP_3477: PP_3477 - Putative secretion type II protein related to protein G, at 3,941,551 to 3,942,030
_3477
PP_3478: PP_3478 - putative Secretion protein, at 3,942,027 to 3,943,892
_3478
Position (kb)
3941
3942
3943 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3940.758 kb on - strand, within PP_3475 at 3940.835 kb on + strand, within PP_3475 at 3940.907 kb on + strand, within PP_3475 at 3940.954 kb on + strand, within PP_3475 at 3940.980 kb on - strand, within PP_3475 at 3941.007 kb on - strand, within PP_3475 at 3941.007 kb on - strand, within PP_3475 at 3941.009 kb on + strand, within PP_3475 at 3941.009 kb on + strand, within PP_3475 at 3941.009 kb on + strand, within PP_3475 at 3941.031 kb on - strand, within PP_3475 at 3941.150 kb on + strand at 3941.150 kb on + strand at 3941.150 kb on + strand at 3941.151 kb on - strand at 3941.151 kb on - strand at 3941.154 kb on + strand at 3941.172 kb on + strand at 3941.172 kb on + strand at 3941.172 kb on + strand at 3941.173 kb on - strand at 3941.173 kb on - strand at 3941.322 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.376 kb on + strand, within PP_3476 at 3941.377 kb on - strand, within PP_3476 at 3941.380 kb on - strand, within PP_3476 at 3941.458 kb on - strand at 3941.603 kb on + strand, within PP_3477 at 3941.603 kb on + strand, within PP_3477 at 3941.733 kb on - strand, within PP_3477 at 3941.733 kb on - strand, within PP_3477 at 3941.776 kb on + strand, within PP_3477 at 3941.811 kb on + strand, within PP_3477 at 3941.855 kb on + strand, within PP_3477 at 3941.882 kb on + strand, within PP_3477 at 3941.883 kb on - strand, within PP_3477 at 3941.920 kb on + strand, within PP_3477 at 3941.921 kb on - strand, within PP_3477 at 3942.104 kb on + strand at 3942.532 kb on - strand, within PP_3478 at 3942.553 kb on + strand, within PP_3478 at 3942.554 kb on - strand, within PP_3478
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs remove 3,940,758 - PP_3475 0.17 +0.3 3,940,835 + PP_3475 0.31 +0.9 3,940,907 + PP_3475 0.45 +0.8 3,940,954 + PP_3475 0.54 -1.5 3,940,980 - PP_3475 0.59 +0.6 3,941,007 - PP_3475 0.64 -0.9 3,941,007 - PP_3475 0.64 +1.4 3,941,009 + PP_3475 0.64 +0.7 3,941,009 + PP_3475 0.64 -1.4 3,941,009 + PP_3475 0.64 +2.1 3,941,031 - PP_3475 0.68 -0.3 3,941,150 + +0.4 3,941,150 + +1.1 3,941,150 + +0.6 3,941,151 - -0.8 3,941,151 - -0.2 3,941,154 + +0.2 3,941,172 + +1.4 3,941,172 + +2.4 3,941,172 + +0.6 3,941,173 - -1.5 3,941,173 - +0.4 3,941,322 + PP_3476 0.39 +0.7 3,941,376 + PP_3476 0.53 -0.6 3,941,376 + PP_3476 0.53 +0.7 3,941,376 + PP_3476 0.53 +0.3 3,941,376 + PP_3476 0.53 +0.4 3,941,376 + PP_3476 0.53 -1.3 3,941,377 - PP_3476 0.53 -1.0 3,941,380 - PP_3476 0.54 +0.9 3,941,458 - -0.8 3,941,603 + PP_3477 0.11 +0.9 3,941,603 + PP_3477 0.11 +0.9 3,941,733 - PP_3477 0.38 +0.5 3,941,733 - PP_3477 0.38 -0.8 3,941,776 + PP_3477 0.47 -2.0 3,941,811 + PP_3477 0.54 -0.4 3,941,855 + PP_3477 0.63 +0.9 3,941,882 + PP_3477 0.69 -0.1 3,941,883 - PP_3477 0.69 +0.8 3,941,920 + PP_3477 0.77 +1.0 3,941,921 - PP_3477 0.77 +1.4 3,942,104 + -1.3 3,942,532 - PP_3478 0.27 -0.4 3,942,553 + PP_3478 0.28 +0.7 3,942,554 - PP_3478 0.28 +1.0
Or see this region's nucleotide sequence