Strain Fitness in Pseudomonas putida KT2440 around PP_3224

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPP_3223 and PP_3224 are separated by 74 nucleotidesPP_3224 and PP_3225 are separated by 15 nucleotidesPP_3225 and PP_3226 overlap by 4 nucleotides PP_3223: PP_3223 - ABC transporter, periplasmic binding protein, at 3,658,246 to 3,659,865 _3223 PP_3224: PP_3224 - Class II aldolase/adducin domain protein, at 3,659,940 to 3,660,734 _3224 PP_3225: PP_3225 - D-isomer specific 2-hydroxyacid dehydrogenase family protein, at 3,660,750 to 3,661,694 _3225 PP_3226: PP_3226 - Acyl-CoA dehydrogenase-related protein, at 3,661,691 to 3,662,833 _3226 Position (kb) 3659 3660 3661Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3658.991 kb on + strand, within PP_3223at 3659.150 kb on + strand, within PP_3223at 3659.151 kb on - strand, within PP_3223at 3659.175 kb on - strand, within PP_3223at 3659.175 kb on - strand, within PP_3223at 3659.259 kb on - strand, within PP_3223at 3659.259 kb on - strand, within PP_3223at 3659.456 kb on + strand, within PP_3223at 3659.457 kb on - strand, within PP_3223at 3659.583 kb on + strand, within PP_3223at 3659.583 kb on + strand, within PP_3223at 3659.583 kb on + strand, within PP_3223at 3659.583 kb on + strand, within PP_3223at 3659.584 kb on - strand, within PP_3223at 3659.584 kb on - strand, within PP_3223at 3659.585 kb on + strand, within PP_3223at 3659.585 kb on + strand, within PP_3223at 3659.585 kb on + strand, within PP_3223at 3659.586 kb on - strand, within PP_3223at 3659.586 kb on - strand, within PP_3223at 3659.712 kb on - strandat 3660.005 kb on + strandat 3660.078 kb on + strand, within PP_3224at 3660.078 kb on + strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.079 kb on - strand, within PP_3224at 3660.278 kb on - strand, within PP_3224at 3660.286 kb on + strand, within PP_3224at 3660.287 kb on - strand, within PP_3224at 3660.301 kb on + strand, within PP_3224at 3660.304 kb on + strand, within PP_3224at 3660.304 kb on + strand, within PP_3224at 3660.305 kb on - strand, within PP_3224at 3660.436 kb on + strand, within PP_3224at 3660.598 kb on + strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.599 kb on - strand, within PP_3224at 3660.637 kb on + strand, within PP_3224at 3660.901 kb on + strand, within PP_3225at 3660.901 kb on + strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3660.902 kb on - strand, within PP_3225at 3661.052 kb on - strand, within PP_3225at 3661.161 kb on + strand, within PP_3225at 3661.339 kb on - strand, within PP_3225at 3661.564 kb on - strand, within PP_3225

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
remove
3,658,991 + PP_3223 0.46 -1.0
3,659,150 + PP_3223 0.56 -3.1
3,659,151 - PP_3223 0.56 +1.4
3,659,175 - PP_3223 0.57 -0.0
3,659,175 - PP_3223 0.57 +2.9
3,659,259 - PP_3223 0.63 -3.3
3,659,259 - PP_3223 0.63 -1.4
3,659,456 + PP_3223 0.75 +0.6
3,659,457 - PP_3223 0.75 -0.4
3,659,583 + PP_3223 0.83 -1.2
3,659,583 + PP_3223 0.83 +1.1
3,659,583 + PP_3223 0.83 +1.1
3,659,583 + PP_3223 0.83 -1.0
3,659,584 - PP_3223 0.83 +1.5
3,659,584 - PP_3223 0.83 -0.9
3,659,585 + PP_3223 0.83 +0.0
3,659,585 + PP_3223 0.83 +0.1
3,659,585 + PP_3223 0.83 +0.8
3,659,586 - PP_3223 0.83 +0.5
3,659,586 - PP_3223 0.83 -0.8
3,659,712 - +0.2
3,660,005 + +1.5
3,660,078 + PP_3224 0.17 -0.9
3,660,078 + PP_3224 0.17 +1.4
3,660,079 - PP_3224 0.17 -0.8
3,660,079 - PP_3224 0.17 +0.7
3,660,079 - PP_3224 0.17 -0.3
3,660,079 - PP_3224 0.17 +0.0
3,660,079 - PP_3224 0.17 -1.3
3,660,278 - PP_3224 0.43 -0.1
3,660,286 + PP_3224 0.44 +0.6
3,660,287 - PP_3224 0.44 -0.6
3,660,301 + PP_3224 0.45 -0.8
3,660,304 + PP_3224 0.46 -0.4
3,660,304 + PP_3224 0.46 -1.0
3,660,305 - PP_3224 0.46 -3.2
3,660,436 + PP_3224 0.62 -0.9
3,660,598 + PP_3224 0.83 -3.8
3,660,599 - PP_3224 0.83 -0.8
3,660,599 - PP_3224 0.83 -0.5
3,660,599 - PP_3224 0.83 -1.3
3,660,599 - PP_3224 0.83 -1.2
3,660,637 + PP_3224 0.88 -2.6
3,660,901 + PP_3225 0.16 -0.8
3,660,901 + PP_3225 0.16 +0.1
3,660,902 - PP_3225 0.16 +2.0
3,660,902 - PP_3225 0.16 +1.6
3,660,902 - PP_3225 0.16 -0.2
3,661,052 - PP_3225 0.32 -0.7
3,661,161 + PP_3225 0.43 -0.9
3,661,339 - PP_3225 0.62 +0.8
3,661,564 - PP_3225 0.86 -1.9

Or see this region's nucleotide sequence